病毒介导的GFP-ATG8在小麦上的表达和在自噬活性监测上的应用

胡蕊洁, 杨向芸, 贾磊, 李玉如, 项月, 岳洁瑜, 王华忠

Virus-mediated expression of GFP-ATG8 for autophagy monitoring in wheat

HU Rui-Jie, YANG Xiang-Yun, JIA Lei, LI Yu-Ru, XIANG Yue, YUE Jie-Yu, WANG Hua-Zhong

图5 融合蛋白GFP-TaATG8a在烟草叶表皮细胞中的定位 从过表达GFP或GFP-TaATG8a的烟草植株叶片背面注射100 μmol L-1 E-64D (+E-64D)或1%溶剂DMSO (-E-64D), 将注射后的叶片剪下置于蒸馏水中并保存于黑暗条件下进行离体饥饿处理。于处理后24 h在共聚焦显微镜下观察表皮细胞中的GFP荧光并照相。标尺为50 μm。

Fig. 5 Localization of GFP-TaATG8a proteins in the leaf epidermal cells of N. benthamiana N. benthamiana leaves expressing GFP or GFP-TaATG8a were injected with 100 μmol L-1 E-64D (+E-64D) or an equal volume of 1% solvent DMSO (-E-64D) from the leaf abaxial side. These leaves were then detached and treated with starvation by keeping them in distilled water under dark conditions. GFP fluorescence in leaf epidermal cells was observed under a laser scanning confocal microscope at 24 hours after starvation treatment. Bar: 50 μm.