病毒介导的GFP-ATG8在小麦上的表达和在自噬活性监测上的应用

胡蕊洁, 杨向芸, 贾磊, 李玉如, 项月, 岳洁瑜, 王华忠

Virus-mediated expression of GFP-ATG8 for autophagy monitoring in wheat

HU Rui-Jie, YANG Xiang-Yun, JIA Lei, LI Yu-Ru, XIANG Yue, YUE Jie-Yu, WANG Hua-Zhong

图7 融合蛋白GFP-TaATG8a在小麦叶肉细胞中的定位 从过表达GFP或GFP-TaATG8a的小麦植株叶片背面主脉切口处注射100 μmol L-1 E-64D (+E-64D)或1%溶剂DMSO (-E-64D), 将注射后的叶片剪下置于蒸馏水中并保存于黑暗条件下进行离体饥饿处理。于处理后24 h在共聚焦显微镜下观察叶肉细胞中的GFP荧光并照相。标尺为20 μm。

Fig. 7 Localization of GFP-TaATG8a proteins in the mesophyll cells of wheat Wheat leaves expressing GFP or GFP-TaATG8a were injected with 100 μmol L-1 E-64D (+E-64D) or an equal volume of 1% solvent DMSO (-E-64D) from prepared cuts on the main leaf veins. These leaves were then detached and treated with starvation by keeping them in distilled water under dark conditions. GFP fluorescence in the mesophyll cells was observed under a laser scanning confocal microscope at 24 hours after starvation treatment. Bar: 20 μm.