| ProBnMAPK7-PA | F: GACGTCGACAATATAATGTCTAATGAGTGAACCAAAC
R: GACCCATGGGCTTTCTTGTCCCTAAACTCAACC | ProBnMAPK7-PA (1612 bp)全长启动子表达载体的构建
Construction of promoter expression vector of full-length ProBnMAPK7-PA (1612 bp) |
| ProBnMAPK7-lPB | F: GACGTCGACAATATAATGTCTAATGAGTGAACCAAAC
R: GACCCATGGTATAAAAAACATGTTTATCTTTTAGGTTG | ProBnMAPK7-lPB (539 bp)启动子表达载体的构建
Construction of promoter expression vector of ProBnMAPK7-lPB (539 bp) |
| ProBnMAPK7-rPB | F: GACGTCGACGTTTGGATGAGAGAGTATTATGGATCCCGG
R: GACCCATGGGCTTTCTTGTCCCTAAACTCAACC | ProBnMAPK7-rPB (1073 bp)启动子表达载体的构建
Construction of promoter expression vector of ProBnMAPK7-rPB (1073 bp) |
| ProBnMAPK7-lPC | F: GACGTCGACGTTTGGATGAGAGAGTATTATGGATCCCGG
R: GACCCATGGAATATGACACGTGGCTTACG | ProBnMAPK7-lPC (212 bp)启动子表达载体的构建
Construction of promoter expression vector of ProBnMAPK7-lPC (212 bp) |
| ProBnMAPK7-rPC | F: GACGTCGACCCACTGTACCCACGTGCTTAGCCGGTTGC
R: GACCCATGGGCTTTCTTGTCCCTAAACTCAACC | ProBnMAPK7-rPC (861 bp)启动子表达载体的构建
Construction of promoter expression vector of ProBnMAPK7-rPC (861 bp) |
| ProBnMAPK7-lPD | F: GACGTCGACCCACTGTACCCACGTGCTTAGCCGGTTGC
R: GACCCATGGCCAGATTGTAATTACAGAAGCTG | ProBnMAPK7-lPD (623 bp)启动子表达载体的构建
Construction of promoter expression vector of ProBnMAPK7-lPD (623 bp) |
| ProBnMAPK7-rPD | F: GACGTCGACGTATGTCGAAATCTTCTTTTCTTTGC
R: GACCCATGGGCTTTCTTGTCCCTAAACTCAACC | ProBnMAPK7-rPD (238 bp)启动子表达载体的构建
Construction of promoter expression vector of ProBnMAPK7-rPD (238 bp) |
| ProBnMAPK7-lPE | F: GACGTCGACCCACTGTACCCACGTGCTTAGCCGGTTGC
R: GACCCATGGTTTCTTACTTCTTTTCTTTTTTCAGAA | ProBnMAPK7-lPE (394 bp)启动子表达载体的构建
Construction of promoter expression vector of ProBnMAPK7-lPE (394 bp) |
引物名称
Primer name | 引物序列
Primer sequences (5°-3°) | 用途
Usage |
| ProBnMAPK7-rPE | F: GACGTCGACGGCTTCTTCATCAAACATAACGTATTGCTTC
R: GACCCATGGCCAGATTGTAATTACAGAAGCTG | ProBnMAPK7-rPE (229 bp)启动子表达载体的构建
Construction of promoter expression vector of ProBnMAPK7-rPE (229 bp) |
| p1305.1 | F: GATTCATTAATGCAGCTGGCACGACAGG
R: GTTGATCGGGTACAGACTAGTTCGTCGG | 启动子表达载体的插入检测
Insertion detection of promoter expression
vector |
| GUS | F: CAACTCGCTGCGTGATGGCATG
R: GATAGGAGTGTCCTCATGTTTGCC | 启动子表达载体的GUS基因检测
Detection of GUS gene in promoter expression vector |
| Hyg | F: GAACTCACCGCGACGTCTGT
R: GGCGTCGGTTTCCACTATCG | 启动子表达载体的Hyg基因检测
Detection of Hyg gene in promoter expression vector |
| pAbAi | F: CTTCCTTCTGTTCGGAGATTACCGAATC
R: TATACATACAGAGCACATGCCTCG | pAbAi-ProBnMAPK7-rPE×3表达载体的检测
Detection of expression vector of pAbAi- ProBnMAPK7-rPE×3 |
| Bait | F: GGCGGATAATGCCTTTAGCGGCTTAACTG
R: CCCGGAATGCAGTGAAGGAAAAGCACCG | ProBnMAPK7-rPE×3整合到Y1Hgold染色体的检测
Detection of ProBnMAPK7-rPE×3 integration in to Y1Hgold chromosome |
| Y1H | F: CTATCTATTCGATGATGAAGATACCCC
R: GCACAGTTGAAGTGAACTTGCGGGGTTTTTC | ProBnMAPK7-rPE的候选上游调控因子检测
Detection of candidate upstream regulators of ProBnMAPK7-rPE |
| AD | F: CTATTCGATGATGAAGATACCCCACCAAACCC
R: AGTGAACTTGCGGGGTTTTTCAGTATCTACGAT | 文库筛选Prey酵母菌落的检测
Detection of Prey yeast cells from library screening |