甘蓝型油菜丝裂原活化蛋白激酶7基因(BnMAPK7)上游调控因子的鉴定 |
王珍, 张晓莉, 孟晓静, 姚梦楠, 缪文杰, 袁大双, 朱冬鸣, 曲存民, 卢坤, 李加纳, 梁颖 |
Identification of upstream regulators for mitogen-activated protein kinase 7 gene (BnMAPK7) in rapeseed (Brassica napus L.) |
WANG Zhen, ZHANG Xiao-Li, MENG Xiao-Jing, YAO Meng-Nan, MIU Wen-Jie, YUAN Da-Shuang, ZHU Dong-Ming, QU Cun-Min, LU Kun, LI Jia-Na, LIANG Ying |
图2 BnMAPK7 (BnaA09g09520D)在甘蓝型油菜不同时期不同组织中的表达模式(A)和逆境响应模式(B)分析 Ro_24h、Ro_48h、Ro_72h分别表示种子萌发24 h、48 h、72 h的胚根; Hy_48h、Hy_72h分别表示种子萌发48 h、72h的下胚轴; Co_24h、Co_48h、Co_72h分别表示种子萌发24 h、48 h、72 h的子叶。Ro_s、Ro_s_f分别表示苗期的温室植株根系、大田植株根系; Le_s、Le_s_f分别表示苗期的温室植株叶片、大田植株叶片。Ro_b、St_b、LeY_b、LeO_b、Bu_b、IT_b分别表示蕾薹期的根系、茎、幼叶、成熟叶片、花蕾、主序顶端。Ro_i、St_i、LeY_i、LeO_i、Ao_i、Cal_i、Pe_i、Pi_i、Sta_i、At_i、Cap_i、IT_i分别表示初花期的根系、茎、幼叶、成熟叶片、花柄、萼片、花瓣、雌蕊、雄蕊、花药、花丝、主序顶端。Ro_f、St_f、LeY_f、LeO_f、Ao_f、Cal_f、Pe_f、Pi_f、Sta_f、At_f、Cap_f、IT_ f分别表示盛花期的根系、茎、幼叶、成熟叶片、花柄、萼片、花瓣、雌蕊、雄蕊、花药、花丝、主序顶端。St_24d表示青荚期花后24 d的茎; LeY_10d、LeY_24d、LeY_30d分别表示青荚期花后10 d、24 d、30 d的幼叶; LeO_10d、LeO_24d、LeO_30d分别表示青荚期花后10 d、24 d、30 d的成熟叶片; Se_10d、Se_24d、Se_30d分别表示青荚期花后10 d、24 d、30 d的种子; Em_21d、Em_30d分别表示青荚期花后21 d、30 d的种胚; SC_21d、SC_30d分别表示青荚期花后21 d、30 d的种皮; En_21d表示青荚期花后21 d的内种皮; Ep_30d表示青荚期花后30 d的外种皮; SP_10d、SP_24d、SP_30d表示青荚期花后10 d、24 d、30 d的荚果皮。St_50d表示成熟期花后50 d的茎; SC_43d、SC_46d分别表示成熟期花后43 d、46 d的种皮; Em_43d、Em_46d分别表示成熟期花后43 d、46 d的种胚; Ra_43d、Ra_46d分别表示成熟期花后43 d、46 d的胚芽; SP_43d、SP_46d分别表示成熟期花后43 d、46 d的荚果皮。 |
Fig. 2 Relative expression patterns of BnMAPK7 (BnaA09g09520D) in different tissues at different stages (A) and stresses responses (B) in Brassica napus Ro_24h, Ro_48h, and Ro_72h represent the root at 24, 48, and 72 hours after seed germination, respectively; Hy_48h and Hy_72h represent the hypocotyl at 48 hours and 72 hours after seed germination, respectively; Co_24h, Co_48h, and Co_72h represent the cotyledon at 24, 48, and 72 hours after seed germination, respectively. Ro_s, Ro_s_f represent the root from seedlings planted in greenhouse and field at the seedling stage, respectively; Le_s, Le_s_f represent the leaf from seedlings planted in greenhouse and field at the seedling stage, respectively. Ro_b, St_b, LeY_b, LeO_b, Bu_b, and IT_b represent the root, stems, young leaf, mature leaf, bud, and the tip of main inflorescence at the bolting stage, respectively. Ro_i, St_i, LeY_i, LeO_i, Ao_i, Cal_i, Pe_i, Pi_i, Sta_i, At_i, Cap_i, and IT_i represent the root, stem, young leaf, mature leaf, anthocallus, calyx, petal, pistil, stamen, anther, capillament, and the tip of main inflorescence at the initial bloom stage, respectively. Ro_f, St_f, LeY_f, LeO_f, Ao_f, Cal_f, Pe_f, Pi_f, Sta_f, At_f, and Cap_f, IT_f represent the root, stem, young leaf, mature leaf, anthocallus, calyx, petal, pistil, stamen, anther, capillament, and the tip of main inflorescence at the full bloom stage, respectively. St_24d represents the stem at 24 days after flowering at the green pod stage; LeY_10d, LeY_24d, and LeY_30d represent the young leaf at 10, 24, and 30 days after flowering at the green pod stage, respectively; LeO_10d, LeO_24d, and LeO_30d represent the mature leaf at 10, 24, and 30 days after flowering at the green pod stage, respectively; Se_10d, Se_24d, and Se_30d represent the seed at 10, 24, and 30 days after flowering at the green pod stage, respectively; Em_21d and Em_30d represent the embryo at 21 days and 30 days after flowering at the green pod stage, respectively; SC_21d and SC_30d represent the seed coat at 21 days and 30 days after flowering at the green pod stage, respectively; En_21d represents the endotesta at 21 days after flowering at the green pod stage; Ep_30d represents the episperm at 30 days after flowering at the green pod stage; SP_10d, SP_24d, and SP_30d represent the silique pericarp at 10, 21, and 30 days after flowering at the green pod stage, respectively. St_50d represents the stem at 50 days after flowering at the mature stage; SC_43d and SC_46d represent the seed coat at 43 days and 46 days after flowering at the mature stage, respectively; Em_43d and Em_46d represent the embryo at 43 days and 46 days after flowering at the mature stage, respectively; Ra_43d and Ra_46d represent the radicle at 43 days and 46 days after flowering at the mature stage, respectively; SP_43d and SP_46d represent the silique pericarp at 43 days and 46 days after flowering at the mature stage, respectively. |