甘蓝型油菜CBF基因家族的鉴定和表达分析

解盼, 刘蔚, 康郁, 华玮, 钱论文, 官春云, 何昕

Identification and relative expression analysis of CBF gene family in Brassica napus L.

XIE Pan, LIU Wei, KANG Yu, HUA Wei, QIAN Lun-Wen, GUAN Chun-Yun, HE Xin

表1 本研究所用qRT-PCR引物

Table 1 Primers for qRT-PCR used in this study

基因名称 Gene name 正向引物序列 Forward sequence (5°-3°) 负向引物序列 Reverse sequence (5°-3°) BnaCBF4-RT GAGACCACGGAAGAGAACGG AGTCGTTATGATTCCATCCAAGTT BnaCBF1-C09-RT CGACTATGGTTGAGGCAGTGAAA AAGTCGTTATGATTCCATCCAAGTT BnaCBF3-RT CCAAGCCGAGTCAGCAAAAT GAGAAACTCAGGTAAATGGGTGTG BnaCBF1-Cnn-RT GAGACGACGGTGGAGGGTG AAGTCATCATTATGTCCCCATTGTA BnaC05g34300D/BnaACTIN GGTTGGGATGGACCAGAAGG TCAGGAGCAATACGGAGC