结合RNA-seq分析和QTL定位筛选甘蓝型油菜萌发期与铝毒胁迫相关的候选基因

王瑞莉, 王刘艳, 雷维, 吴家怡, 史红松, 李晨阳, 唐章林, 李加纳, 周清元, 崔翠

Screening candidate genes related to aluminum toxicity stress at germination stage via RNA-seq and QTL mapping in Brassica napus L.

WANG Rui-Li, WANG Liu-Yan, LEI Wei, WU Jia-Yi, SHI Hong-Song, LI Chen-Yang, TANG Zhang-Lin, LI Jia-Na, ZHOU Qing-Yuan, CUI Cui

表1 测序质量分析

Table 1 Quality analysis of sequencing data

样品名称 Sample name S0 R0 ST RT 总数 Total 原始读数Raw reads 48,585,792 55,778,592 45,860,428 47,308,644 197,533,456 干净读数Clean reads 44,642,424 51,491,214 41,819,768 43,518,768 181,472,174 干净碱基Clean data (bp) 6,696,363,600 7,723,682,100 6,272,965,200 6,527,815,200 27,220,826,100 N (%) 0.001385 0.00138 0.001418 0.00137 Q20 (%) 97.57 97.59 97.72 97.43 Q30 (%) 94.14 94.22 94.43 93.74