结合RNA-seq分析和QTL定位筛选甘蓝型油菜萌发期与铝毒胁迫相关的候选基因

王瑞莉, 王刘艳, 雷维, 吴家怡, 史红松, 李晨阳, 唐章林, 李加纳, 周清元, 崔翠

Screening candidate genes related to aluminum toxicity stress at germination stage via RNA-seq and QTL mapping in Brassica napus L.

WANG Rui-Li, WANG Liu-Yan, LEI Wei, WU Jia-Yi, SHI Hong-Song, LI Chen-Yang, TANG Zhang-Lin, LI Jia-Na, ZHOU Qing-Yuan, CUI Cui

表2 用于qRT-PCR的候选差异表达基因引物

Table 2 Primers of candidate differentially expressed genes used for qRT-PCR

基因名称 Gene name 正向引物 Forward primer (5°-3°) 反向引物 Reverse primer (5°-3°) BraACTIN7 GGAGCTGAGAGATTCCGTTG GAACCACCACTGAGGACGAT BnaA03g47720D ACAAAGCAATGCACTCTTTAGG GTTGTTGAAGACTCTGCAGTTT BnaC03g62130D AACAAAGACAAACCTGGGACTA CTTGTTGATCGAACGGTAATCG BnaA09g02030D CTTCTGCTCAAGGCTCT GGGTTTCCCAATGTTAG BnaA03g03740D GAAGTATGTGAGGATGGAGAGG GATCTTCTCCAAATCAGCGTTC BnaA03g53200D GCCATTGTTGTATATTGGGCAT TGATGTGTATGAAACAAGCAGC BnaC03g65590D ACGTTGAGCCAAACAGAGTG CTATTATCGTAGACACCGGACG