结合RNA-seq分析和QTL定位筛选甘蓝型油菜萌发期与铝毒胁迫相关的候选基因

王瑞莉, 王刘艳, 雷维, 吴家怡, 史红松, 李晨阳, 唐章林, 李加纳, 周清元, 崔翠

Screening candidate genes related to aluminum toxicity stress at germination stage via RNA-seq and QTL mapping in Brassica napus L.

WANG Rui-Li, WANG Liu-Yan, LEI Wei, WU Jia-Yi, SHI Hong-Song, LI Chen-Yang, TANG Zhang-Lin, LI Jia-Na, ZHOU Qing-Yuan, CUI Cui

表3 R0 vs RT和S0 vs ST中的KEGG显著富集分析

Table 3 KEGG pathway significantly overrepresented in R0 vs RT and S0 vs ST

KEGG编号KEGG ID KEGG术语 KEGG term 基因数目 Genes number 比值 Ratio (%) 矫正后的P值 P-value R0 vs RT bna00940 苯丙烷生物合成Phenylpropanoid biosynthesis 55 21.32 3.81844E-21 bna00500 淀粉和蔗糖代谢Starch and sucrose metabolism 27 10.47 2.29236E-06 bna00910 氮素代谢Nitrogen metabolism 13 5.04 1.81952E-05 bna00270 半胱氨酸和蛋氨酸代谢CySteine and methionine metabolism 21 8.14 0.000110181 bna04016 MAPK信号通路-植物MAPK signaling pathway-plant 23 8.91 0.000143365 bna00010 糖酵解/糖异生Glycolysis/Gluconeogenesis 21 8.14 0.000268893 bna00130 泛醌和其他萜类醌生物合成 Ubiquinone and other terpenoid-quinone biosynthesis 11 4.26 0.001478368 bna00380 色氨酸代谢Tryptophan metabolism 12 4.65 0.003486812 bna00360 苯丙氨酸代谢Phenylalanine metabolism 10 3.88 0.006641539 KEGG编号KEGG ID KEGG术语 KEGG term 基因数目 Genes number 比值 Ratio (%) 矫正后的P值 P-value bna00460 氰基氨基酸代谢Cyanoamino acid metabolism 12 4.65 0.006641539 bna00350 酪氨酸代谢Tyrosine metabolism 9 3.49 0.007588613 bna04075 植物激素信号转导Plant hormone signal transduction 34 13.18 0.007588613 bna00052 半乳糖代谢Galactose metabolism 10 3.88 0.009316627 S0 vs ST bna00940 苯丙烷生物合成Phenylpropanoid biosynthesis 97 31.80 5.89634E-43 bna04016 MAPK信号通路-植物MAPK signaling pathway-plant 38 12.46 9.09024E-08 bna00910 氮素代谢Nitrogen metabolism 16 5.25 2.123E-05 bna00460 氰基氨基酸代谢Cyanoamino acid metabolism 19 6.23 0.000428636 bna04075 植物激素信号转导Plant hormone signal transduction 52 17.05 0.001727744 bna00520 氨基糖和核苷酸糖代谢 Amino sugar and nucleotide sugar metabolism 27 8.85 0.004361711 bna04626 植物-病原体相互作用Plant-pathogen interaction 35 11.48 0.004455032 bna00040 戊糖和葡糖醛酸相互转化 Pentose and glucuronate interconversions 21 6.89 0.005458802