结合RNA-seq分析和QTL定位筛选甘蓝型油菜萌发期与铝毒胁迫相关的候选基因

王瑞莉, 王刘艳, 雷维, 吴家怡, 史红松, 李晨阳, 唐章林, 李加纳, 周清元, 崔翠

Screening candidate genes related to aluminum toxicity stress at germination stage via RNA-seq and QTL mapping in Brassica napus L.

WANG Rui-Li, WANG Liu-Yan, LEI Wei, WU Jia-Yi, SHI Hong-Song, LI Chen-Yang, TANG Zhang-Lin, LI Jia-Na, ZHOU Qing-Yuan, CUI Cui

表4 QTL和转录组结合后筛出可能与铝毒胁迫相关的候选基因

Table 4 Candidate genes possibly involved in aluminum poison coercion using QTL and RNA-Seq techniques

性状 Trait 位点 QTL 甘蓝型油菜基因编号 Gene ID in B. napus 基因登录号 Gene accession 调节 Regulated RT:fpkm ST:fpkm 基因注释 Gene annotations 相对发芽势RGV Up 19.6 0.87 BnaA01g31480D AT3G10720 Up 58.3 16.8 植物转化酶/果胶甲基酯酶抑制剂超家族 Plant invertase/Pectin methyl esterase inhibitor superfamily BnaA01g30490D AT3G12580 Up 176 56.5 热激蛋白70heatshockprotein70,hsp70 BnaA01g28900D AT3G15450 Up 264 30 Al具有YGL和LRDR图案的铝诱导蛋白质 Al-induced protein with YGL and LRDR patterns BnaA01g27670D AT3G16690 Up 11.7 0.47 结蛋白MtN3家族蛋白Nodin MtN3 family protein qRGV-A03-1 BnaA03g50730D AT3G48000 Down 0.08 13.5 乙醛脱氢酶2B4Acetaldehyde dehydrogenase 2B4 BnaA03g50130D AT4G30420 Up 20.9 4.05 结蛋白MtN21 /EamA样转运蛋白家族蛋白 Nodin MtN21 /EamA-like transporter family protein BnaA03g50050D AT4G30270 Up 36.2 9.65 木葡聚糖内葡聚糖酰化酶/水解酶 (XTH) Xyloglucan endoglucanase/hydrolase (XTH) BnaA03g47720D AT4G26010 Up 6.4 0.7 过氧化物酶超家族蛋白Peroxidase superfamily protein BnaA03g47250D AT4G25260 Up 36.6 4.89 植物转化酶/果胶甲基酯酶抑制剂超家族 Plant invertase/Pectin methyl esterase inhibitor superfamily BnaA03g46060D AT4G23590 Up 11 0.16 酪氨酸转氨酶家族蛋白Tyrosine transaminase family protein BnaA03g45950D AT4G00430 Up 18.7 2.9 跨膜蛋白CTransmembrane protein c BnaA03g44820D AT4G21680 Down 3.21 4.06 硝酸盐转运体Nitrate transporter BnaA03g43140D AT4G17550 Up 10.3 0.05 主要促进因子超家族蛋白质Major promoter superfamily proteins BnaA03g42860D AT4G16820 Up 6.25 0.62 α/β-水解酶超家族蛋白质α/β-hydrolase superfamily protein BnaA03g42310D AT4G15960 Up 17.6 4.18 α/β-水解酶超家族蛋白质α/β-hydrolase superfamily protein BnaA03g40990D AT3G50830 Down 0.31 17.7 冷调节413等离子体Cold conditioning 413 plasma BnaA03g40380D AT5G61600 Up 173 31.6 乙烯响应因子104 (ERF104) Ethylene response factor 104 (ERF104) qRGV-A03-2 BnaA03g09460D AT5G59530 Down 1.94 14.8 2-氧代戊二酸和铁依赖性加氧酶超家族蛋白 2-oxoglutarate and iron-dependent oxygenase superfamily proteins BnaA03g09400D AT5G59550 Up 42.4 10.6 锌指(C3HC4型无名指)家族蛋白 Zinc finger (C3HC4 ring finger) family protein BnaA03g09290D AT5G59730 Up 27.3 5.38 外胚层亚单位exo70家族蛋白 Exoderm subunit exo70 family protein BnaA03g08870D AT5G60530 Up 10.7 0.26 晚期胚胎发生丰富蛋白/ LEA蛋白 Late embryogenesis rich protein/LEA protein BnaA03g08820D AT5G60660 Up 44.5 0.84 质膜内在蛋白2 Plasma membrane intrinsic protein 2 BnaA03g07790D AT5G19855 Down 3.53 6.86 伴侣蛋白RbcX Companion protein RbcX BnaA03g07660D AT5G19600 Down 2.83 21.8 硫酸盐转运蛋白Sulfate transporter BnaA03g03740D AT5G12020 Up 99.8 18.3 17.6 kD二类热激蛋白(HSP17.6II) 17.6 kD class ii heat shock protein (HSP17.6II) qRGV-C03 BnaC03g08610D AT5G17820 Up 14.7 0.78 过氧化物酶超家族蛋白Peroxidase superfamily protein 相对发芽率RGR qRGR-C04 BnaC04g10740D AT2G34500 Up 55.9 12.3 细胞色素P450,家族710,亚家族A,多肽1 (CYP710) Cytochrome P450, family 710, subfamily A, polypeptide 1 (CYP710) BnaC04g08480D AT2G36780 Down 0.9 23.3 UDP-糖基转移酶超家族蛋白 UDP-glycosyltransferase superfamily protein BnaC04g07650D AT2G37820 Up 10.1 0.22 富含半胱氨酸/组氨酸结构域家族蛋白 Protein rich in cysteine/histidine domain family BnaC04g06480D AT2G38940 Down 3.11 182 磷酸盐转运蛋白Phosphate transporter BnaC04g06140D AT2G39430 Up 33.4 5.44 抗病反应(定向蛋白样)家族蛋白 Disease resistance response (directed protein-like) family protein 相对根长RRL qRRL-A03-2 BnaA03g53200D AT4G35180 Down 0.46 0.31 赖氨酸/组氨酸转运蛋白7 (LHT7) Lysine/histidine transporter 7 (LHT7) BnaA03g52830D AT4G34410 Up 17.2 2.6 氧化还原反应转录因子1 (RRTF1) Redox transcription factor 1 (RRTF1) qRRL-A09 BnaA09g02040D AT4G12550 Up 37.1 0 生长素诱导的根培养Root culture induced by auxin BnaA09g02030D AT4G12550 Up 470 34.5 生长素诱导的根培养1Auxin-induced root culture 1 qRRL-C03 BnaC03g65590D AT4G35060 Down 4.57 6.67 重金属转运/解毒超家族蛋白 Heavy metal transport/detoxification superfamily protein BnaC03g65200D AT4G23550 Up 13.6 1.28 WRKY29家族WRKY29 familyWRKY29 family wrky 29 family BnaC03g62130D AT4G23700 Up 256 70.6 阳离子/氢离子交换器17 (CHX17) Cation/hydrogen ion exchanger 17 (CHX17) 相对芽长RBL qRBL-C08 BnaC08g17880D AT1G18300 Up 115 28.1 nudix水解酶同源物(NUDT) Nudix hydrolase homologue (NUDT) BnaC08g16940D AT1G16390 Up 1.89 0.05 有机阳离子/肉碱转运蛋白Organic cation/carnitine transporter 相对干重RDW qRDW-A09-1 BnaA09g30120D AT1G23090 Up 0.04 0.66 硫酸盐转运蛋白(AST) Sulfate transporter (AST) BnaA09g29940D AT4G13390 Up 38.4 3.17 富含脯氨酸的伸展蛋白样家族蛋白 Proline-rich extension-like family proteins qRDW-A10-2 BnaA10g25970D AT5G04250 Down 7.13 22.2 半胱氨酸蛋白酶超家族蛋白 Cysteine protease superfamily protein