抗根肿病甘蓝型油菜新品种华油杂62R的选育

李倩, Nadil Shah, 周元委, 侯照科, 龚建芳, 刘珏, 尚政伟, 张磊, 战宗祥, 常海滨, 傅廷栋, 朴钟云, 张椿雨

Breeding of a novel clubroot disease-resistant Brassica napus variety Huayouza 62R

LI Qian, Nadil Shah, ZHOU Yuan-Wei, HOU Zhao-Ke, GONG Jian-Fang, LIU Jue, SHANG Zheng-Wei, ZHANG Lei, ZHAN Zong-Xiang, CHANG Hai-Bin, FU Ting-Dong, PIAO Zhong-Yun, ZHANG Chun-Yu

附表1 用于遗传背景筛选的多态性分子标记

Table S1 Polymorphic molecular markers used for genetic background screening

染色体 Chr. 标记名称 Marker name 物理位置 Physical location (Mb) 正向引物 Forward primer (5°-3°) 反向引物 Reverse primer (5°-3°) A01 niab_ssr113 366,648 CAAAAAGTTGCGGTCAATCT CCTCCAAAGCTCAATCACTG sau_um356 2,137,110 CATCTTCGTCTCTCCATCACCT GTATGGTAGGAGGAGAGTTCGCT cnu_ssr134 4,481,886 TCTCTTTGCCATCGTCGTTTC CCCCTCAAACTGAGCAGTCAA niab_ssr032 7,684,077 TTCTCCCCATCCTCTCATCTTA ACCCACAACCAACAAAATCTTC cnu_mBRPGM0190 9,861,961 GAGATCCAATAGCGAGCACA TGTGTTATCGGGTGAAGTGG 染色体 Chr. 标记名称 Marker name 物理位置 Physical location (Mb) 正向引物 Forward primer (5°-3°) 反向引物 Reverse primer (5°-3°) A01 ACMP00456 13,955,674 CTCTACAAGCCGCAGAGAGA CATCCACCACAGAGATTGCT ACMP00617 15,044,996 AACTCAATGCTCTTCGCTCA CCTTCTGCTGCTTCCAAGAT ACMP00297 16,999,515 CAGATATTGCCCAAACACCA CTTCGCTTCTGAGCTTTCCT sau_um586 20,719,752 AGGGTGATGATGATGTACAGGAG CTTCGTTAATTCTTCCACCCAG cnu_ssr125 26,899,174 TCCAAGTGAAGGATAATGCTCGT TGTACAATGGGGATGTTGTGG A02 sau-um619 1,404,160 GAGAGCTCACTTGTGCTTCTTGT CCGATCAAGTTATTCGCTCTCT cnu_ssr116 3,025,687 GCCATGGTGGTGGGATTAGA TGGTCGAACGTGTGTCGATAAT niab_ssr143 5,142,784 GATATGTTTCCAAACAAGTCAA AAATTCGACCCCTTTTCG sau_um434 7,390,390 AGCTAGGGATGAGAAGAAGACCG TCTTTGTGTCCGTCAAGAGTCC cnu_ssr036 10,214,155 AGCACAGTCAAAACTCAGAATGA AGGCATGCGCTCTCTATCTC cnu_mBRPGM1391 11,315,073 GGTTTAGAGTGGTGCGGAAT CCGGGCCTAAAGTAATCAAA cnu_mBRPGM0182a 14,588,355 TGGAGTAGAAGCGTCGTCTG CCTTCCTCCTCTCAGTTCCA cnu_mBRPGM1527a 16,911,280 GCAAGCACGGAGACTAAACA GACGGTGAAGCTGTACGAAG cnu_mBRPGM0813 18,824,804 GTTCCATAGGGCGTTCACAT CCGATGATTCTCTCAATCAGG cnu_ssr447 21,870,396 TGGTGTCAAACGGACAGAAA ATACTCGGCTCAAACCGTCA CB10416 23,408,087 GCTGTTGCTGTAGGTTTGA GAGCCAGCGTTGATAAGA A03 niab_ssr115 1,049,474 CGGTGTATACCGAACGAGAA AAACCCAATCAACCCCTTTA ACMP00292 2,920,902 GGGTTGCTGGTTTAGCTGTT TGAATCCGCTGAACTCTCTG cnu_mBRPGM0240 5,317,208 GAGGGAAAGAGGACAATACGA TCATCGAGAAACGAAGGGTA cnu_ssr173 8,444,756 TGTATTCCATTATTTCCGACTAACCT CCGCATTTTAAAAACGTGAGAAA cnu_ssr290 9,860,984 CGATTTTGCCATTGTCTAAGC TGAAGACACGTTGGTTGAACA ACMP00755 11,006,524 GTCATCGCAAGAGGACAAGA AAAGCTCCATCAAAGCACCT cnu_ssr098 14,357,780 TGCGACCCAAGTAGGTGAAAC TGTCTCTCGCTCATTCATCCAA cnu_ssr327 17,867,509 TTCTTGACCAAAAGAATCATGG CTAACACGGGGAAAAGCAGA ACMP00186 19,379,932 GCCTCCCTGACTTGTACCTC TTCAATGCGCCAGTTAAGAC ACMP00410 20,585,363 CTCAAAGCATGAACGTGGAC CCTCCCTTGATTTGTGGAGT sau_um146 22,875,333 CTCGCAAAATCCCTTCTTCG CATATCGCTCGAGTTGCAGA cnu_ssr526 23,351,679 TCCGAGAAGCACACAAAGAA TGACCATTTTCTGCCATTCA TCR079 23,692,426 TGACGTTCAATCAAAGCCTGA TTTAGCAATCAAATGCAAATTCAA cnu_ssr492 23,747,774 TCGAGGTGGTTACAATCCAA CAATGCGGATCTACCTCTCA cra_id011 24,345,511 GTCGGATTTCTTTCTACACG TGAACCTATCTTCCTCAACG cra_ssr015 24,345,899 CGGCCTCCGGAAATTTATTA TGGGAGGACCTCTCTCTTCTT Cra_RT1 24,351,487 ACGTAAAGAAGCTGACCGGAGAC AGGCTTAACAACAGTTCCAGATT cra_ssr017 24,355,996 GTGTGACCGCACTGTTGTTT AGTTTGACCCAAACGCATAA sau_um026 25,648,956 AGTGGCTCCCAGGAGGATAATA CTTGGAGAAGAGAAACTTGGGC cnu_ssr241 27,414,883 AATGCTGTGTCCATGACCAA CGGGCATCCACCTAATTTGT At2g35530 29,980,641 CAGAGTAACTGGTTATGCCCGTC CAATAGGGATAAACCTGGAGACAG ACMP00563 31,183,993 CGGAGATGGGATTAAGGAGA AAAGATCGTGTGGGTGGAAT A04 BrID10929 251,988 CAATTTGGGAAGACAGTTCT GATTCGTTGATATAAGGCCA BrID10321 2,417,875 TGTGTTTCCTAGTGTGTTGG ATCAGTCTGAGGGTTCATCA BrID101249 3,990,390 TTGCATAGCACATGTAGGAG GAACGTCTACTTATGGAGAACA BrID10645 7,441,204 GCAGAGGAAAACAATCAGAC AAGCGTCGACTTGAAATCT ACMP00073 8,674,696 TTCAACCACACCGACAAACT CTGACGGAGTCCCTGTACCT ACMP00356 11,811,660 TGAGGTCTACAGCCCAAGTG AATGGAGATCGTGTGCAAAG 染色体 Chr. 标记名称 Marker name 物理位置 Physical location (Mb) 正向引物 Forward primer (5°-3°) 反向引物 Reverse primer (5°-3°) A04 ACMP00744 12,860,981 CCTGTTCCAATCCCATTCTT AGATCCCTGACGGGTTTATG ACMP00281 15,028,874 TCGACTTTGACGAAGACTGG AGTCCTCATGGCATCAAACC hri_Mbrms195 17,105,559 AATACTTTCTGAAGTTGTCCGCTAA AACCTACGCAAGATGCTTCTACTT cnu_ssr005 18,776,905 AGGAGTCTCGTTCCGTGAGA TTGAAATTAAGTCGAGCAAACAA A05 cnu_ssr387 236,456 ACTCGAACCATTCTGGCAAA GGAACGACTTCCTCCCGTAT niab_ssr017 2,501,334 GGTTAAGCAGACGATGGAAGTAA TATAGGGGAAATCATCTCAAGCA cnu_ssr381 4,003,395 TGCTTTTAACCAAACTCAAACG TGCAGAGAGGCAAGTTTCAA sau_um392 5,882,422 GCCAGTTTCGTCTTCTTCTCTG GAAGTCACACCCCCATCTCTATC BrID90500 6,762,400 ATGGGCTTTGTTTGGGTAAA TGAAAGAGACAAGCTGGCAA BrID10825 9,949,084 CAATGTGTTCGATGGAATG TAGTGTGCGAACAGATTCAG ACMP00841 13,132,010 CAGTGGCAACAACATCAACA CTTCACATCGTCTCCACCAC BrID101239 13,226,921 TCCACACAGAAGATAACTGGT GAGATGACATTTTCGCTGAC cnu_ssr458 16,415,511 GGGGTGAATCTTGGATGAGG CTGACGGATTCCCAACGAAT sau_um366 18,549,687 TTCTCCTCGTATCACCACTCCT GCCTACGTCTTCTACAGCGAGAT niab_ssr082 20,079,421 CATTTCCCCGTGACTATCTG CGTCTTCATCTCAATCTCGC sau_um551 21,619,807 GTCCATCTCCTACCTGCTCCA GTTTTGAGCCGAATAATGGTTG hri_mBRMS007 23,268,922 AAATTGTTTCTCTTCCCCAT GTGTTAGGGAGCTGGAGAAT A06 BrID10395 1,513,120 GCTGACATGTACCTTTTGAA CATCTAAGACCGAGTCAAGC sau_um278 3,092,702 GAGAACAAGAGGAGGACGCTT CCGGAGGCAAGTATCCATAAG niab_ssr134 5,621,445 CGCAGCCTTTTGCTTCT TTGCTCTCCTGCAGCTTG cnu_mBRPGM1016a 6,689,917 TGGAGATGGCTGTTGTTGAT AGCAGATGTCGGGAATAACC niab_ssr049 7,659,576 GAGGAATTAACGGCGTCTTG CAGTCGCCACTACCTGGTTT ACMP00739 10,965,852 GGTGACTGTTCCTCATCAGC ATCCCTATCCAAACCTCTCG BrID10847 14,198,854 TCATTGCCTTACTTTGTGAC CTGACACAGGTGAATCAACA BrID10849 14,265,494 AAAGATCTGTGGAATCATGG GGCAAACATGGGTTGATA BrID10627 15,630,795 AACACAGATCCAATCTAGCG GTCCTTAGGCCAAGCATT ACMP00692 17,268,545 CGAGTTGCAGAGCCAAGTAG AACGTAACGCTTCCTCTTCC sau-um121 18,555,402 GAACCTAACGAAAGGCACCAC AGTGAGGGTAGACAGGGAGAGAG cnu_ssr220 21,896,695 ATCAGAACCGAATCCGACCA CAATGGTTGCAATGTTATTTGGA sau-um415 22,971,940 ACGCAAAGAGAGCGAAAGAGTC GGTCTTAATCGCATGGAATCCG sau-um616 23,447,329 ATTACCTATTGACCCCACACCAC GACGTAGAACAAGTGAGAAGGGA A07 ACMP00261 1,018,274 AAGCCTCGACTTTGGAAGAT ATCTCCGTCTGGTCTCGTCT At2g06510 2,516,307 GATCGGGTTAAGTCAGGACA ATGGTCTCCATGTTCAGCAC ACMP00785 4,675,451 TGGCTCTGTTTCCTTCTCCT AAGGGATTGATCGGAACTTG BrID10283 8,743,728 CGGTTAGGTCGTAACTCGTA CTCTTCATACGCAAGTCTTT BrID10487 11,667,757 TCCCTTACAAGTTCATGGAT ATGGTAGCAACAAAACCAGT nia_m063 12,010,219 GAAGAAACTCGGTGGGGAGT AAAGAGTTCCGAAAATGGGC cnu_ssr511 14,701,383 TGTGGACGAGAAACTGAGGA TGAGATACTGGTGCGTGTGG cnu_ssr044 16,404,302 TGTTTTGATCTTTACTGTTTTTGGA AATGTTTTTATATCACTATTGCCAAAT cnu_ssr048 18,226,238 TTCTCCATGCTGTTCAATTCAC CATGAAAAATCGACCTTATTCCA cnu_ssr516 20,127,600 ACTTGCCTTAGCCAGCAGCG AAGATTTTGTGTTGTGGTCTGGTGA cnu_ssr1566 22,103,544 GTCAGACTCGGATGGCTTG TAGGAGCAGTTGGTTCAGCA A08 ACMP00659 1,364,408 CCGCCTCAATCTCTAGCTTT CTCGTTCACCACCTCTGCTA ACMP00551 2,716,097 CAGCAATGGTGGACATCTCT AACAAGACCGGAACCATCTC 染色体 Chr. 标记名称 Marker name 物理位置 Physical location (Mb) 正向引物 Forward primer (5°-3°) 反向引物 Reverse primer (5°-3°) A08 BrID90197 4,203,640 TGTTCAAACTTCCCACCCTC GTGAGCCCAAAAGTTCCTGA BrID10839 6,657,012 AGGAACAATACCCATTTGTG GGTGTTTGTGTGTTCGGTA BrID101199 7,356,083 ATGATGGAGATGGACATTTG TGTATCGGCAGAAGAATCTC sau_um192 10,064,084 TCCCTCCTCTCTACGTCTTCTTC CTTCTCTGTAACGGGCTTTGAC sau_um044 14,603,121 CGATTCATCCATCCATCACC CTATAGGACCGATCCCGTCTTT cnu_ssr432 16,431,751 CAAACCTCGTCCTAAGCAGAA ACCTGAAGATGACCCAGACG sau_um077 17,469,120 CTGATCCTCGAAGAAGACAGTGC CTTCATGCACTTGAGGAGTCG cnu_ssr176 18,202,437 TGTAAGTCACGTTCGGTTTGCT AGGCATGTATGGAGATGTAGAGTGA BRMS-198 21,521,373 CGAGAGCAGTTAGGAAGCTTATAGA AGAGATACTCTGTCCTCCACCTCTT A09 nia_m010 1,815,031 GGTTGACGTCTCATTGTGTTCTT TAGCTTTGCTCACTTTTCACTCC sau_um219 3,482,038 CGCAGCTTCCTCTGTATTGCTA GGCTCTCACCAGAGTCAAGTCTA cnu_ssr157 5,671,382 CCGCAGTTGATCCATTAGCC ACGCTGCATCCACATGAAAC sau_um368 6,944,590 AGCCCCGTCTCTTTCACTGTAT GATCTAGGGTCTCGTCGACTTTG nia_m022 10,330,441 CTCTCGTCTCGGAGGATCTAAA GTGAGAGTGGTTGCTGAGTGAG sau_um019 13,593,016 GGTCCTGCCATTCCTATTCTCT CATGCCACGTCAGCAATATG sau_um138 16,538,853 CGCACACCATTTCCACAAAC GAGATGAATGTGCGTCTCCTG ACMP00188 18,078,993 GATTCCTCTCCACGACCATT TCTCCCAAATCGGTTCTTTC sau_um101 21,178,401 GATCTTATCGTGCCCATTGC CTCCTCATAGGGCTCCTTTTTC cnu_ssr598 24,440,145 TTCACCGTCTGCTCTTATCG CTGCTCCCATACGATCCACT cnu_ssr016 27,461,366 GGTGAATGGAATCTTGTCTTGA CCCAACAATCCCAGAAACAC cnu_ssr119 30,057,081 ACACCTACTTGTTTCCATCCAAAT CGGGTATTTGCGTTGTTTCC sau_um187 33,166,753 GTCCTCCTCAACCTCATCATCA AGTCGAGAGTAACGGGAAGAGAG sau_um105 36,739,828 CCTTTCTAATGGGAAGCGGTAG CTCCCTCTTCGAATTGACTCAC A10 sau_um126 2,528,134 AGAACACGCTCCTAACCATCAG TAGCTACGAGGCCTTAGAGGGTA niab_ssr034 4,065,070 GTGCAAGTCAGTGCCAAAGA CTCGGTGGTTGAGTGAAGGT sau_um433 7,037,138 AAGAGTCCACAGCAGGAGATTG GGGATGAGAAAAAGACAGGTGG sau_um216 10,127,674 CTTTCTTCTCTCCGTCGTTCC AAGGTTAGGGTTAGAGAAGCCG niab_ssr123 11,313,024 GGATCTAGAAACCCCTTCACA ATCTTGTGTCGGGCAGATAA Nniab_ssr122 12,936,001 ACTTCTCCGGCTGGATACTC CCGTTTAAACTTGCGTTTGT niab_ssr009 15,004,910 TTCCCAAGCTTGCTGGTACT GAGATTTCCCTCGCTTGATG sau_um310 15,341,225 TCTTTTCCATCTCTCTACCATCATC CCTATGAGAGGAAGACCGAGACT