小麦TaPP2-A13基因的表达响应逆境胁迫并与SCF复合体接头蛋白TaSKP1相互作用
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孟钰玉, 魏春茹, 范润侨, 于秀梅, 王逍冬, 赵伟全, 魏新燕, 康振生, 刘大群
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TaPP2-A13 gene shows induced expression pattern in wheat responses to stresses and interacts with adaptor protein SKP1 from SCF complex
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MENG Yu-Yu, WEI Chun-Ru, FAN Run-Qiao, YU Xiu-Mei, WANG Xiao-Dong, ZHAO Wei-Quan, WEI Xin-Yan, KANG Zhen-Sheng, LIU Da-Qun
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表1 本实验中涉及的引物信息
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Table 1 Primer information used in this study
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引物名称
Primer name | 上游引物序列
Forward primer sequence
(5'-3') | 下游引物序列
Reverse primer sequence
(5'-3') | 退火温度
Annealing
temperature (℃) | | TaPP2-A13-F/R | AATGGCGGAATCCCTCGTG | CGGGTTTTGGACAAGAATGG | 58.0 | | TaPP2-A13-qPCR-F/R | ATCGATGATCGGCGGTATTG | AGTGGAGCCGGAAGAGAAGG | 59.5 | | TaGAPDH-qPCR-F/R | CTGCCTTGCTCCTCTTGCTAA | CTTGATGGAAGGACCAGCAAC | 59.5 | | TaPP2-A13-His-F/R | gctgatatcggatccgaattcATGGGGGC GGGGGCTTCG | tgcggccgcaagcttgtcgacTTACTTGCGTATGC ACTCCTCG | 60.0 | | TaPP2-A13-GFP-F/R | atacaccaaatcgactctagaATGGGGGC GGGGGCTTCG | catggtaccggatccactagtCTTGCGTATGCACT CCTCGG | 60.0 | | TaPP2-A13-BD-F/R | CGgaatccATGGGGGC | ggatccTTACTTGCGTATGCACT | 60.0 | | AD-F/R | TAATACGACTCACTATAGGGCG | AGATGGTGCACGATGCACAG | 56.0 | | TaPP2-A13-NE-F/R | cccaggcctactagtggatccATGGGGGC GGGGGCTTCG | agcggtaccctcgaggtcgacCTTGCGTATGCACT CCTCGG | 60.0 | | TaSKP1-FLAG-F/R | gagaacacgggggactctagaATGGCGG CCGCGGGAGAC | cgtcctaggcttaagtctagaCTCAAAGGCCCACT GGTTCTC | 60.0 | | TaPP2-A13-HA-F/R | agacttaagcctaggacgcgtATGGGGGC GGGGGCTTCG | AtcgtatgggtacatacgcgtCTTGCGTATGCACT CCTCGG | 60.0 |
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