小麦TaPP2-A13基因的表达响应逆境胁迫并与SCF复合体接头蛋白TaSKP1相互作用
孟钰玉1(
), 魏春茹1, 范润侨1, 于秀梅1,2,*(), 王逍冬2, 赵伟全2, 魏新燕2, 康振生3, 刘大群2
), 魏春茹1, 范润侨1, 于秀梅1,2,*(), 王逍冬2, 赵伟全2, 魏新燕2, 康振生3, 刘大群2
TaPP2-A13 gene shows induced expression pattern in wheat responses to stresses and interacts with adaptor protein SKP1 from SCF complex
MENG Yu-Yu1(), WEI Chun-Ru1, FAN Run-Qiao1, YU Xiu-Mei1,2,*(), WANG Xiao-Dong2, ZHAO Wei-Quan2, WEI Xin-Yan2, KANG Zhen-Sheng3, LIU Da-Qun2
), WEI Chun-Ru1, FAN Run-Qiao1, YU Xiu-Mei1,2,*(), WANG Xiao-Dong2, ZHAO Wei-Quan2, WEI Xin-Yan2, KANG Zhen-Sheng3, LIU Da-Qun2
图8. Co-IP分析本氏烟草叶片中TaPP2-A13与TaSKP1的互作
将烟草总蛋白提取物与带有anti-HA的beads一起孵育, 通过anti-FLAG和anti-HA进行免疫沉淀物分析。
Fig. 8. Interaction of TaPP2-A13 and TaSKP1 in Nicotiana benthamiana leaves by Co-IP
Total protein extracts of N. benthamiana were incubated with anti-HA affinity beads, the immunoprecipitates were analyzed by immunoblotting with anti-FLAG and anti-HA antibodies.
