玉米籽粒突变体smk7的表型分析和基因定位

蒋成功, 石慧敏, 王红武, 李坤, 黄长玲, 刘志芳, 吴宇锦, 李树强, 胡小娇, 马庆

Phenotype analysis and gene mapping of small kernel 7 (smk7) mutant in maize

JIANG Cheng-Gong, SHI Hui-Min, WANG Hong-Wu, LI Kun, HUANG Chang-Ling, LIU Zhi-Fang, WU Yu-Jin, LI Shu-Qiang, HU Xiao-Jiao, MA Qing

表1 基因定位引物序列

Table 1 Primer sequences for gene mapping used in the study

引物 Primer 正向序列 Forward sequence (5°-3′) 反向序列 Reverse sequence (5′-3′) In4.3 AACGCATCATCCTATGTCCAAC GGGTGAAGCCAGCCATTATTT In1.9 ATGGTACGATCAACATAAAGGGAA GGCGTCACCGAAGAAATACAC In0.83 GCAAGAAGCACCAGCCCT CGAGCGAAAGAAAGGAATGT In1.16 ACATGACCCACGATCCAGACA TGCAGCCACTCTCCTTATGGT SNP1 TTAGGGTGGAGTTGCTTCGC TTATGAATGAAGCACGGAAATGA SNP2 TTAGGGTGGAGTTGCTTCGC TTATGAATGAAGCACGGAAATGA SNP3 AAGGAATGGAGGCTTGGGTT ACAGCCGCCTTCGGATTC SNP4 AGCGGTCCTTGACTTTATTTGA CATTACACGACCAATACAGCCA SNP5 TGGCTTTTCATACCCTCCTCC CCTTCGCTGTGACTTGGATGT