白菜型油菜srb多室性状的遗传分析与分子鉴定

杨阳, 李淮琳, 胡利民, 范楚川, 周永明

Genetic analysis and molecular characterization of multilocular trait in the srb mutant of Brassica rapa L.

YANG Yang, LI Huai-Lin, HU Li-Min, FAN Chu-Chuan, ZHOU Yong-Ming

图2 BrCLV3基因结构和氨基酸序列比对 a: BrCLV3基因结构以及突变体srb与野生型材料间的碱基变异; 黑色方框表示编码区; 序列比较可以发现srb与野生型间存在2个单核苷酸突变和1个ATAT的插入/缺失突变。b: 白菜型油菜及拟南芥中CLV3同源基因的氨基酸序列比对; 箭头为CLV3信号肽序列剪切位点, 下划线为C端保守结构域CLE motif, 红色标识突变的氨基酸。

Fig. 2 BrCLV3 gene structure and amino acid sequences alignment a: BrCLV3 gene structure and natural variations between the alleles from WT and srb; black boxes represents the coding regions; there are two single nucleotide substitutions and one ATAT insertion/deletion in BrCLV3 gene regions between WT and srb. b: alignment of the amino acid sequences of CLV3 from B. rapa (srb, WT, ml4, Chiifu-401, and Arabidopsis); the putative signal sequence cleavage site is indicated by arrow, the conserved domain (CLE motif) at its C-terminus is underlined, and the amino acid changes in srb and ml4 are highlighted by the red highlighted font.