白菜型油菜srb多室性状的遗传分析与分子鉴定

杨阳, 李淮琳, 胡利民, 范楚川, 周永明

Genetic analysis and molecular characterization of multilocular trait in the srb mutant of Brassica rapa L.

YANG Yang, LI Huai-Lin, HU Li-Min, FAN Chu-Chuan, ZHOU Yong-Ming

图4 转化35S::Brclv3Asp12可以部分恢复拟南芥clv3-2多室突变表型 野生型拟南芥ler为两室角果(a, b)和正常花序(q, r), clv3-2突变体的角果主要为5~6室(i~l)和异常增大的花序顶端分生组织(m, n), 35S::Brclv3Asp12转化clv3-2突变体产生了部分表型恢复单株, 其角果为2~6室(c~l)和较为正常花序(o, p)。部分恢复转基因家系中BrCLV3转录水平的RT-PCR检测(s)以及单株角果心皮数统计分析(t)。小写字母表示在0.05水平下差异显著。标尺为0.5 mm。

Fig. 4 Phenotypes of 35S::Brclv3Asp12 transgenic plants in the clv3-2 background The phenotypes of bilocular siliques (a, b) and normal inflorescence (q, r) in ler, multilocular siliques with 5-6 locular (i-l) and enlarged inflorescence (m, n) in clv3-2, and 35S::Brclv3Asp12 transgenic plants with partly rescue phenotypes, including 2-6 carpels (c-l) and normal inflorescence (o, p). Gene expression (s) and phenotype analysis of carpel numbers per plant (t) in five independent 35S::Brclv3Asp12 transgenic lines, which showed partially rescued phenotypes; the data and error bars represent the mean ± SD (n ≥ 15 plants for each line). Bars superscripted by lowercase letters indicate significant differences at the 0.05 probability level. Bar = 0.5 mm.