GBS高密度遗传连锁图谱定位甘蓝型油菜粉色花性状

周新桐, 郭青青, 陈雪, 李加纳, 王瑞

Construction of a high-density genetic map using genotyping by sequencing (GBS) for quantitative trait loci (QTL) analysis of pink petal trait in Brassica napus L.

ZHOU Xin-Tong, GUO Qing-Qing, CHEN Xue, LI Jia-Na, WANG Rui

图7 两亲本花瓣中花色相关基因可变剪切视图 A: BnaA07g15980D在黄花亲本62花瓣中转录本剪切视图; B: BnaA07g15980D在粉花亲本77花瓣中转录本剪切视图; C: BnaA07g17500D在黄花亲本62花瓣花瓣中转录本剪切视图; D: BnaA07g17500D在粉花亲本77花瓣中转录本剪切视图。灰色五边形为外显子, 白色区域为内含子, 连线表示不同剪接方式。图片包含4个区域, 第1部分为注释文件基因模型, 第2部分是测序结果, 第3部分为测序结果与注释文件比较基因剪切模型(带有代表性的isoform同源蛋白异构体), 第4部分为测序文件中支持外显子reads数目。左下角、右下角数字为该基因的起始位点和终止位点。

Fig. 7 Splice graphs for genes related to petal color between 77 and 62 A: splice graph for BnaA07g15980D in 62 petals; B: splice graph for BnaA07g15980D in 77 petals; C: splice graph for BnaA07g17500D in 62 petals; D: splice graph for BnaA07g17500D in 77 petals. The gray pentagons represent exons, the white region represent introns, the lines between them represent different ways of splicing. The picture is split into four parts, the first part is the gene model from the annotation file, the second part is the sequencing result, the third part is the gene splicing model between the sequencing result and the annotation file (with a representative protein isoform), the fourth part is the number of reads supporting each exon in the sequencing file. The numbers in the lower left and right corners represent the start and stop sites of the gene, respectively.