DNA甲基化参与调控马铃薯响应干旱胁迫的关键基因挖掘

李鹏程, 毕真真, 孙超, 秦天元, 梁文君, 王一好, 许德蓉, 刘玉汇, 张俊莲, 白江平

Key genes mining of DNA methylation involved in regulating drought stress response in potato

LI Peng-Cheng, BI Zhen-Zhen, SUN Chao, QIN Tian-Yuan, LIANG Wen-Jun, WANG Yi-Hao, XU De-Rong, LIU Yu-Hui, ZHANG Jun-Lian, BAI Jiang-Ping

表1 实时荧光定量PCR所用的引物

Table 1 Primers of real-time quantitative PCR

基因 Gene 正向引物 Forward primer (5'-3') 反向引物 Reverse primer (5'-3') Actin AGGAGCATCCTGTCCTCCTAA CACCATCACCAGAGTCCAACA PGSC0003DMG400001333 CTCAATTGCACCTACTAAGCAC GTGATATTCTTGCATGCAGAGG PGSC0003DMG400005108 GTTGTGTTCTTGGTTTTAGGCT GTGGCATATTCATGGGCATAAG PGSC0003DMG400005112 TCGAATTCTTGTCAACCTGGTA CCTGGTGGATCATAATTGCAAG PGSC0003DMG400005116 CACAATGAAGCTCGTAGACAAG CACATACTCTACCTGCTTGACA PGSC0003DMG400017231 CACGAATTCCAAGAAAAGCAGT CCCCAAATCTTCAAACACAACT PGSC0003DMG400023921 CAACAAATGTGCTAGTGGGAAA TGTTTCCTGAAGGAGCATAGTT PGSC0003DMG400002176 GCAGTCAAGTCAAGAAATATCTACC TGAACACGTACAAGTAAGTGGA PGSC0003DMG400004977 GATTGATGATGTGAAGAGTGCC TGTCATAATGAAGGCCTTGAGT PGSC0003DMG400011012 AATTTTTGTGTGTGGAGGGATG TATTCATTTGAGAGTAGCCGCA PGSC0003DMG400016722 AACTAACAGGAACCACACTG GTTGTGTCCCATTTTAGGGA PGSC0003DMG400020253 GCTGGAAGGTTCATGAAAGATG GGAATGGATATTCTCAATGGCG PGSC0003DMG400024232 GAGCAGGATTTAGCCGTTTTAG AGGTGCAATTACTCCGTAGAAA PGSC0003DMG400006270 ATGCAATTGCTGGTGCTTATAG AGCCTGATAGCAAGTAACAGTT PGSC0003DMG400030038 ATGAAGTTTAGGGGTGACTCAG TACACAAACTTCCCGTATGTGA PGSC0003DMG400030624 TGCTGAAAGTTCATATCCTGGT GAAGTGTCTTGATGCATAGCAG PGSC0003DMG403001316 GATGCTGTGAATCACCCAAATT GATGGTGAACGTATACAACACG PGSC0003DMG401000287 CTTCATTTGACTGGCCTCATTC TCATCAAAAGCACAACCAAGAG PGSC0003DMG401023603 TTAACTGATGAACCGACTTGGA CAATTGTGAGACCAATCGAGAC PGSC0003DMG400013765 TAAGTGCCAGAAGATTGGTCAT TACATCTCTTGCTCCTCAATCG PGSC0003DMG400019274 CTGGGCTTTATAGGTATCGTGT ATATGAAGTTGAATTGAGGCGC PGSC0003DMG400020139 AGCAACAAAAGACCTTCATCAC CATGCTCACTTTAACGTAGCTC PGSC0003DMG400026261 GGATTTGAATTCGAGACGTCTG TCGTTGTAGAAATCATCGCAAC