谷子SiPRR37基因对光温、非生物胁迫的响应特点及其有利等位变异鉴定

贾小平, 李剑峰, 张博, 全建章, 王永芳, 赵渊, 张小梅, 王振山, 桑璐曼, 董志平

Responsive features of SiPRR37 to photoperiod and temperature, abiotic stress and identification of its favourable allelic variations in foxtail millet (Setaria italica L.)

JIA Xiao-Ping, LI Jian-Feng, ZHANG Bo, QUAN Jian-Zhang, WANG Yong-Fang, ZHAO Yuan, ZHANG Xiao-Mei, WANG Zhen-Shan, SANG Lu-Man, DONG Zhi-Ping

表1 特异性引物

Table 1 Specific primers

引物名称 Primer name 引物序列 Primer sequences (5°-3°) 预期片段大小 Expected fragment size (bp) 用途 Purpose PRR37-1 F: TGACAACGACGAGGACG R: CGTTAGCAATCTCCGTGT 1295 克隆SiPRR37 Cloning SiPRR37 PRR37-2 F: CCATTTGCTGACTCGCCTAC R: TCCTGTCCCGCCTTGAT 1586 PRR37-3 F: AAGGCTCCAATGGCAGTAG R: GAACCAGCGAAGGAAGAATC 1267 SiActin F: GGCAAACAGGGAGAAGATGA R: GAGGTTGTCGGTAAGGTCACG 229 内参荧光定量 Internal reference fluorescence quantification RtPRR37 F: CACCACTTTCGTCTACCTCTT R: CTGGCATCTCTTCTAACGG 91 SiPRR37荧光定量 SiPRR37 fluorescence quantification