马铃薯热激转录因子HsfA3基因的克隆及其耐热性功能分析

唐锐敏, 贾小云, 朱文娇, 印敬明, 杨清

Cloning of potato heat shock transcription factor StHsfA3 gene and its functional analysis in heat tolerance

TANG Rui-Min, JIA Xiao-Yun, ZHU Wen-Jiao, YIN Jing-Ming, YANG Qing

表1 本研究所使用的引物

Table 1 Primer sequences used in this study

引物编号 Primer ID. 名称 Name 引物序列 Primer sequence (5′-3′) 用途 Function P1-F CloneHsfA3-F GCGCTCGAGATGAACCCATTTGATAAAAATCAAG StHsfA3的克隆, 酶切位点用下画线标出 P1-R CloneHsfA3-R GTCACGCGTCTAAAAACTATCATTCTTTGGCTG Gene cloning of StHsfA3; the restriction sites are underlined P2-F HsfA3002-F GGGTAATATCCGGAAACCTCCT 转基因验证 P2-R HsfA3002-R GCTTTCTCCATTTCTACCCCAAG Transgenic verification P3-F DLHsfA3-F CGGAAGTTCTTCTGGATCATCTG 荧光定量分析 P3-R DLHsfA3-R CCATCTGCTTCTGTCTTTGTTCT qRT-PCR analysis P4-F DLsHsp3219-F CGTCCAATGTCAGTGTTGGTGG 荧光定量分析 P4-R DLsHsp3219-R CAGTGCCTTGGTTGTTTCCTCC qRT-PCR analysis P5-F DLHsp70-F GTGGCAGTAATGGAAGGTGGGA 荧光定量分析 P5-R DLHsp70-R TTCTCCGGATTCACCACCGATT qRT-PCR analysis P6-F EF1α-F CTGTTAAGGATCTGAAGCGTGGT 荧光定量分析, EF1α为内参基因 P6-R EF1α-R AATGTGGGAAGTGTGGCAGTCG qRT-PCR analysis; EF1α is used as the reference gene