马铃薯热激转录因子HsfA3基因的克隆及其耐热性功能分析

唐锐敏, 贾小云, 朱文娇, 印敬明, 杨清

Cloning of potato heat shock transcription factor StHsfA3 gene and its functional analysis in heat tolerance

TANG Rui-Min, JIA Xiao-Yun, ZHU Wen-Jiao, YIN Jing-Ming, YANG Qing

图4 16个物种HsfA3的系统发育树 利用ClustalX 2.1和MEGA 4.0软件构建来自16个物种的Hsf A3氨基酸序列的系统发育树, 重复计算次数设为1000。各物种名称缩写及NCBI数据库中的序列号如下: MdHsfA3: 苹果(XP_008391748.1); ZjHsfA3: 枣(XP_015898712.1); CsHsfA3: 柑橘(XP_006481891.1); AtHsfA3: 拟南芥(NP_195992.2); VrHsfA3: 豇豆(XP_014511066.1); GmHsfA3: 大豆(XP_003541445.1); PeHsfA3: 杨树(XP_011016541.1); NtHsfA3: 烟草(XP_016473897.1); CaHsfA3: 辣椒(XP_016542894.1); SlHsfA3: 番茄(NP_001234854.1); OsHsfA3: 水稻(XP_015623061.1); ZmHsfA3: 玉米(NP_001147968.1); SiHsfA3: 狗尾草(XP_004952622.1); BdHsfA3: 二穗短柄草(XP_003575055.1); HvHsfA3: 大麦(AEB26588.1)。

Fig. 4 Neighbor-joining phylogenetic tree of HsfA3s from 16 plant species The Neighbor-joining phylogenetic tree of HsfA3 amino acid sequences from 16 plant species was constructed by ClustalX 2.1 and MEGA 4.0, which was bootstrapped over 1000 cycles. Abbreviations for each species and database accession numbers are as follows: MdHsfA3: Malus domestica (XP_008391748.1); ZjHsfA3: Ziziphus jujube (XP_015898712.1); CsHsfA3: Citrus sinensis (XP_006481891.1); AtHsfA3: Arabidopsis thaliana (NP_195992.2); VrHsfA3: Vigna radiata var. radiate (XP_014511066.1); GmHsfA3: Glycine max (XP_003541445.1); PeHsfA3: Populus euphratica (XP_011016541.1); NtHsfA3: Nicotiana tabacum (XP_ 016473897.1); CaHsfA3: Capsicum annuum (XP_016542894.1); SlHsfA3: Solanum lycopersicum (NP_001234854.1); OsHsfA3: Oryza sativa Japonica Group (XP_015623061.1); ZmHsfA3: Zea mays (NP_001147968.1); SiHsfA3: Setaria italica (XP_004952622.1); BdHsfA3: Brachypodium distachyon (XP_003575055.1); HvHsfA3: Hordeum vulgare (AEB26588.1).