马铃薯热激转录因子HsfA3基因的克隆及其耐热性功能分析

唐锐敏, 贾小云, 朱文娇, 印敬明, 杨清

Cloning of potato heat shock transcription factor StHsfA3 gene and its functional analysis in heat tolerance

TANG Rui-Min, JIA Xiao-Yun, ZHU Wen-Jiao, YIN Jing-Ming, YANG Qing

图5 StHsfA3过表达载体的结构示意图(A)、过表达载体的酶切验证(B)、转基因马铃薯植株的再生(C)、StHsfA3过表达转基因植株的PCR检测(D)、不同植株中StHsfA3表达量的qRT-PCR检测(E) A: 带有酶切位点的35S::StHsfA3载体示意图。B: M: 1 kb plus DNA marker; P: 含有pBA002-StHsfA3载体的农杆菌质粒; 1, 2: 含有pBA002-StHsfA3载体的农杆菌质粒酶切结果。C: a. 预培养; b. 芽分化; c. 生根筛选; d. 盆栽苗。D: M: DNA 2000 marker; WT: 非转基因植株, 作为阴性对照; +: pBA002-StHsfA3农杆菌质粒, 作为阳性对照; L1~L5: 转基因株系。E: 不同植株中StHsfA3的表达量测定。**表示在0.01水平差异显著。

Fig. 5 Vector construction of 35S::StHsfA3 (A), enzyme digestion identification of 35S::StHsfA3 vector (B), regeneration of transgenic potato (C), PCR identification of StHsfA3 overexpression transgenic plant lines (D), and qRT-PCR determination of StHsfA3 in WT and StHsfA3 overexpression transgenic plant lines (E) Schematic diagram of 35S::StHsfA3 vector with site of restriction enzymes in Fig. A. M: 1 kb plus DNA marker; P: pBA002-StHsfA3 plasmid; 1, 2: enzyme digestion identification of pBA002-StHsfA3 plasmid in Fig. B. a: preincubation in darkness; b: bud formation; c: selection of transgenic lines; d: plants in pot in Fig. C. M: DNA 2000 marker; WT: non-transgenic potato plant, used as a negative control; +: pBA002-StHsfA3 plasmid, used as a positive control; L1-L5: Transgenic lines in Fig. D. The expression levels of StHsfA3 were determined in different plants in Fig. E. **: significantly different at the 0.01 probability level.