马铃薯热激转录因子HsfA3基因的克隆及其耐热性功能分析

唐锐敏, 贾小云, 朱文娇, 印敬明, 杨清

Cloning of potato heat shock transcription factor StHsfA3 gene and its functional analysis in heat tolerance

TANG Rui-Min, JIA Xiao-Yun, ZHU Wen-Jiao, YIN Jing-Ming, YANG Qing

图6 不同温度处理下非转基因植株与StHsfA3过表达转基因植株叶片中的相对含水量(A)和丙二醛含量(B) WT: 非转基因植株; L: StHsfA3过表达转基因株系(L1、L3、L5三个株系); RWC: 相对含水量; MDA content: 丙二醛含量; 正常生长温度: 22℃; 高温胁迫: 35℃; 处理时间: 8 h; 图中的值是3个独立试验的结果, 每个试验3个重复, 以平均值±标准误表示。根据Duncan’s多重测试, 柱形图上不同小写字母表示P < 0.05的显著性差异水平。

Fig. 6 RWC (A) and MDA (B) content in the leaves of non-transgenic plants and StHsfA3 overexpression transgenic plants under different temperature treatments WT: non-transgenic plants; L: StHsfA3 overexpression transgenic plant lines; RWC: relative water content; MDA: malondialdehyde; temperature treatments: 22℃ (optimal temperature) and 35℃ (heat stress) for eight hours. Values are means ± SE of three independent experiments with three replicates in each experiment. Bars with different lowercase letters are significantly different at P < 0.05 according to Duncan’s multiple range tests.