马铃薯热激转录因子HsfA3基因的克隆及其耐热性功能分析

唐锐敏, 贾小云, 朱文娇, 印敬明, 杨清

Cloning of potato heat shock transcription factor StHsfA3 gene and its functional analysis in heat tolerance

TANG Rui-Min, JIA Xiao-Yun, ZHU Wen-Jiao, YIN Jing-Ming, YANG Qing

图7 不同温度处理下非转基因植株(WT)和转基因植株(L)叶片中StHsfA3 (A)、StHsp26-CP (B)和StHsp70 (C)的表达水平 WT: 非转基因植株; L: StHsfA3过表达转基因株系(L1、L3、L5三个株系); 正常生长温度: 22℃; 高温胁迫: 35℃; 处理时间: 2 h; 图中的值是2次试验重复的结果, 每个试验3个生物学重复, 以平均值±标准误表示。根据邓肯多重测试, 柱形图上不同小写字母表示P < 0.05的显著性差异水平。

Fig. 7 Expression levels of StHsfA3 (A), StHsp26-CP (B), and StHsp70 (C) in the leaves of non-transgenic plants (WT) and transgenic plant lines (L) under different temperature treatments WT: non-transgenic plants; L: StHsfA3 overexpression transgenic plant lines; temperature treatments: 22℃ (optimal temperature) and 35℃ (heat stress) for two hours. Values are means ± SE of two experimental replicates with three biological replicates in each experiment. Bars with different lowercase letters are significantly different at P < 0.05 according to Duncan’s multiple range tests.