水稻氮高效、耐冷基因OsGRF4功能标记的开发及其利用

孙平勇, 张武汉, 张莉, 舒服, 何强, 彭志荣, 邓华凤

Development and application of functional marker for high nitrogen use efficiency and chilling tolerance gene OsGRF4 in rice

SUN Ping-Yong, ZHANG Wu-Han, ZHANG Li, SHU Fu, HE Qiang, PENG Zhi-Rong, DENG Hua-Feng

图1 OsGRF4基因功能标记设计策略 SNP3为功能性多态位点, PF和PR为外引物, DR和XR为反向内引物, 分别与碱基AA和TC匹配。分别在DR和XR的3′端引入错配碱基C开发了DMR和XMR。箭头表示扩增方向。

Fig. 1 Strategy of functional markers design for OsGRF4 SNP3 indicates functional polymorphic sites; PF and PR are outer primers; DR and XR are reverse inner primers, which matching the bases AA and TC, respectively. A mismatch base C was introduced in the 3′ end of DR and XR to develop DMR and XMR. The arrows indicate amplification direction of the primers.