玉米杂交种纯度鉴定SNP核心引物的确定及高通量检测方案的建立
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王蕊, 施龙建, 田红丽, 易红梅, 杨扬, 葛建镕, 范亚明, 任洁, 王璐, 陆大雷, 赵久然, 王凤格
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Identification of SNP core primer and establishment of high throughput detection scheme for purity identification in maize hybrids
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WANG Rui, SHI Long-Jian, TIAN Hong-Li, YI Hong-Mei, YANG Yang, GE Jian-Rong, FAN Ya-Ming, REN Jie, WANG Lu, LU Da-Lei, ZHAO Jiu-Ran, WANG Feng-Ge
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表3 玉米杂交种纯度鉴定SNP核心引物信息表
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Table 3 SNP core primer information for purity identification in maize hybrids
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引物编号 Primer ID | 引物名称 Primer name | 染色体 Chromosome | 染色体位置 Chromosome position | 双亲互补率 Parental complementary rate (%) | 最小等位 基因频率 MAF | 多态信息 含量 PIC | MSNP01 | MG002 | 1 | 8974336 | 51.1 | 0.50 | 0.38 | MSNP06 | MG049 | 1 | 289408285 | 58.0 | 0.46 | 0.37 | MSNP09 | MG064 | 2 | 109563976 | 55.8 | 0.34 | 0.35 | MSNP12 | MG072 | 2 | 186339948 | 53.6 | 0.47 | 0.37 | MSNP14 | MG092 | 3 | 27775731 | 62.3 | 0.47 | 0.37 | MSNP16 | MG101 | 3 | 118083714 | 65.6 | 0.49 | 0.38 | MSNP20 | MG135 | 4 | 20077010 | 51.6 | 0.39 | 0.36 | MSNP22 | MG148 | 4 | 128874466 | 46.9 | 0.33 | 0.35 | MSNP25 | MG175 | 5 | 13402375 | 53.6 | 0.45 | 0.37 | MSNP30 | MG207 | 5 | 204879476 | 53.7 | 0.39 | 0.36 | MSNP31 | MG215 | 6 | 39822979 | 62.4 | 0.50 | 0.38 | MSNP36 | MG240 | 6 | 141476300 | 51.1 | 0.43 | 0.37 | MSNP38 | MG270 | 7 | 126677146 | 47.5 | 0.33 | 0.35 | MSNP42 | MG279 | 7 | 155821714 | 50.3 | 0.42 | 0.37 | MSNP45 | MG293 | 8 | 20978845 | 51.4 | 0.45 | 0.37 | MSNP48 | MG311 | 8 | 118299376 | 56.5 | 0.36 | 0.35 | MSNP52 | MG347 | 9 | 104695670 | 60.4 | 0.42 | 0.37 | MSNP53 | MG349 | 9 | 127197714 | 61.5 | 0.50 | 0.38 | MSNP56 | MG361 | 10 | 39960289 | 57.6 | 0.48 | 0.38 | MSNP58 | MG369 | 10 | 109396730 | 53.3 | 0.50 | 0.38 |
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