玉米杂交种纯度鉴定SNP核心引物的确定及高通量检测方案的建立

王蕊, 施龙建, 田红丽, 易红梅, 杨扬, 葛建镕, 范亚明, 任洁, 王璐, 陆大雷, 赵久然, 王凤格

Identification of SNP core primer and establishment of high throughput detection scheme for purity identification in maize hybrids

WANG Rui, SHI Long-Jian, TIAN Hong-Li, YI Hong-Mei, YANG Yang, GE Jian-Rong, FAN Ya-Ming, REN Jie, WANG Lu, LU Da-Lei, ZHAO Jiu-Ran, WANG Feng-Ge

图1 测试引物分型效果图 A, B, C, F为CTAB法分型效果; D, E为快提法分型效果。A, D为引物MG279, 在CTAB法和快提法均具有清晰、紧密的分型效果; B, E为引物MG304, 该位点使用CTAB法能够正常分型, 但快提法无法正常分型; C为引物MG262, KASP引物分型效果为单态; F为引物MG067, 分型效果较散且数据缺失率较高。图中每1个点代表1个反应孔的结果, 其中蓝色和红色点分别代表2种纯合基因型, 绿色点代表双亲互补基因型, 粉色点代表未采集基因分型, 黑色点代表空白对照。

Fig. 1 Genotyping of tested primers A, B, C, and F are the genotyping of CTAB method; D and E are the genotyping of fast extraction method. A and D are primer MG279, which have clear and close genotyping effect both by CTAB method and rapid extraction method; B and E are primer MG304, which has normal genotyping by CTAB method, but abnormal genotyping by rapid extraction method; C is primer MG262, of which KASP primer genotyping effect is monomorphic; F is primer MG067, of which KASP primer genotyping effect is wide and has high missing data rate. Each point represents the result of one reaction well, of which blue and red points represent two homozygous genotype, green points represent parental complementary genotype, pink points represent unused genotype and black points represent non-template control.