玉米杂交种纯度鉴定SNP核心引物的确定及高通量检测方案的建立

王蕊, 施龙建, 田红丽, 易红梅, 杨扬, 葛建镕, 范亚明, 任洁, 王璐, 陆大雷, 赵久然, 王凤格

Identification of SNP core primer and establishment of high throughput detection scheme for purity identification in maize hybrids

WANG Rui, SHI Long-Jian, TIAN Hong-Li, YI Hong-Mei, YANG Yang, GE Jian-Rong, FAN Ya-Ming, REN Jie, WANG Lu, LU Da-Lei, ZHAO Jiu-Ran, WANG Feng-Ge

图3 待测样品京科968纯度鉴定分型图(快速提取法, 引物MG072) 图中每1个点代表1个单株的检测结果, 其中蓝色和红色点分别代表2种纯合基因型, 绿色点代表双亲互补基因型, 黑色点代表空白对照, 三角形点代表亲本自交系样品。

Fig. 3 Genotyping of purity identification of Jingke 968 (fast extraction method, primer MG072) Each point represents the result of single seed, of which blue and red points represent two homozygous genotype, green points represent parental complementary genotype, black points represent non-template control and triangle points represent parental inbred lines samples.