新型抗广谱性除草剂草甘膦转基因油菜的创制及其鉴定

李杰华, 端群, 史明涛, 吴潞梅, 柳寒, 林拥军, 吴高兵, 范楚川, 周永明

Development and identification of transgenic rapeseed with a novel gene for glyphosate resistance

LI Jie-Hua, DUAN Qun, SHI Ming-Tao, WU Lu-Mei, LIU Han, LIN Yong-Jun, WU Gao-Bing, FAN Chu-Chuan, ZHOU Yong-Ming

表1 本研究所用引物序列

Table 1 Primer sequences used in this study

引物名称 Primer name 引物序列 Primer sequence (5°-3°) 引物名称 Primer name 引物序列 Primer sequence (5°-3°) EPSPS-F ATTAGCGCTAGGGACGTGAG P1 CTCTAGCCAATACGCAAACCGCC EPSPS-R ATACGCTCCCACATCCTGTC P3 TGTTGTGTGGAATTGTGAGCGGA 35SP-1 GAAACCGGTTACAGGCAAATG SP2 CAGGTGGAGAACGTCGGTACGACT 35SP-2 AGAGGCGGTTTGCGTATT SP3 GGGTTTCGCTCATGTGTTGAGCA 35ST-1 ATAACACATTGCGGACGTTT BnACT-qRTF CTGGAATTGCTGACCGTATGAG 35ST-2 AGCCGGTGCTTGATAACT BnACT-qRTR ATCTGTTGGAAAGTGCTGAGGG 35SP-2 AGAGGCGGTTTGCGTATT EPs-qRTF CAGGGATGGACTCGTTATCAC p130-35sF CAAGTGGATTGATGTGATAACATG EPs-qRTR GAGGGTCTTCGCAGTCGT p130-35sR GTAGAGAGAGACTGGTGATTTCAGC EPSPS6 F GGCTTCTTAGTGGGCCTTTC Iva-EPSPS-F ATTAGCGCTAGGGACGTGAG EPSPS7 F CATCCGCTTTACGACGAAACT Iva-EPSPS-R ATACGCTCCCACATCCTGTC EPSPS2 F GAACTGAGCATGAATGGCATAAA P5 ATGTGTGAGTAGTTCCCAGATAAGG