新型抗广谱性除草剂草甘膦转基因油菜的创制及其鉴定

李杰华, 端群, 史明涛, 吴潞梅, 柳寒, 林拥军, 吴高兵, 范楚川, 周永明

Development and identification of transgenic rapeseed with a novel gene for glyphosate resistance

LI Jie-Hua, DUAN Qun, SHI Ming-Tao, WU Lu-Mei, LIU Han, LIN Yong-Jun, WU Gao-Bing, FAN Chu-Chuan, ZHOU Yong-Ming

图2 转I. variabilis EPSPS基因植株的PCR阳性检测结果 A: 利用引物对EPSPS-F/EPSPS-R检测目的基因I. variabilis EPSPS的PCR结果; B: 利用引物对35SP-1/35SP-2检测CaMV 35S启动子元件的PCR结果; C: 利用引物对35ST-1/35ST-2检测CaMV 35S终止子元件的PCR结果。M为2 kb DNA marker; P为pTGH质粒(阳性对照); N为转化受体J9707 (阴性对照); 1~12为T0代部分转化植株, 编号分别为EPS-1~EPS-12。

Fig. 2 PCR identification of positive transgenic plants of the I. variabilis EPSPS gene A: PCR identification of the target gene I. variabilis EPSPS by primer pair EPSPS-F/EPSPS-R; B: PCR identification of the CaMV 35S promoter by primer pair 35SP-1/35SP-2; C: PCR identification of the CaMV 35S terminator by primer pair 35ST-1/35ST-2. M: 2 kb DNA marker; P: the positive control of pTGH-1plasmid; N: the negative control of J9707; 1-12: the T0 transgenic plants from EPS-1 to EPS-12.