新型抗广谱性除草剂草甘膦转基因油菜的创制及其鉴定

李杰华, 端群, 史明涛, 吴潞梅, 柳寒, 林拥军, 吴高兵, 范楚川, 周永明

Development and identification of transgenic rapeseed with a novel gene for glyphosate resistance

LI Jie-Hua, DUAN Qun, SHI Ming-Tao, WU Lu-Mei, LIU Han, LIN Yong-Jun, WU Gao-Bing, FAN Chu-Chuan, ZHOU Yong-Ming

图3 转I. variabilis EPSPS基因T0代阳性植株的Southern blot拷贝数检测结果 A: Southern blot检测结果。杂交探针为位于目的基因上的EPSPS探针, 基因组DNA采用Hind III酶切。M: DNA marker; 1~31: 转基因阳性植株; 1: EPS-1; 3: EPS-2; 12: EPS-7; 29: EPS-3; 31: EPS-6; N: J9707 (阴性对照)。B: 不同拷贝数的单株统计。

Fig. 3 Determination of the I. variabilis EPSPS transgenic copy number by Southern blotting analysis in the T0-positive transgenic plants A: detection of Southern blot analysis. The hybrid probe is on the I. variabilis EPSPS gene; the genomic DNA was digested with Hind Ш before electrophoresis. M: DNA marker; 1-31: the positive transgenic plants; 1: EPS-1; 3: EPS-2; 12: EPS-7; 29: EPS-3; 31: EPS-6; N: J9707 (negative control). B: summary of the copy number of transgenic plants.