新型抗广谱性除草剂草甘膦转基因油菜的创制及其鉴定

李杰华, 端群, 史明涛, 吴潞梅, 柳寒, 林拥军, 吴高兵, 范楚川, 周永明

Development and identification of transgenic rapeseed with a novel gene for glyphosate resistance

LI Jie-Hua, DUAN Qun, SHI Ming-Tao, WU Lu-Mei, LIU Han, LIN Yong-Jun, WU Gao-Bing, FAN Chu-Chuan, ZHOU Yong-Ming

图6 EPS-2、EPS-6和EPS-7等转基因家系的转化体特异性PCR检测结果 A: EPS-2转基因家系不同世代材料的转化体特异性PCR检测结果, 检测所用引物为EPSPS-2 F和P5; B: EPS-6转基因家系不同世代材料的转化体特异性PCR检测结果, 检测所用引物为EPSPS6 F和P5; C: EPS-7转基因家系不同世代材料的转化体特异性PCR检测结果, 检测所用引物为EPSPS7F和P5。其中, M为2 kb DNA marker; P为pTGH-1质粒(阳性对照); N为转化受体J9707 (阴性对照); 1为T0代植株; 2~4为T1代抗性植株; 5~7为T2代抗性植株; 8~10为T3代抗性植株。

Fig. 6 PCR confirmation of the T-DNA insertion in the transgenic lines of EPS-2, EPS-6, and EPS-7 with specific primers A: PCR confirmation of the T-DNA insertion in the transgenic line of EPS-2 with specific primer pair EPSPS2 F/P5. B: PCR confirmation of the T-DNA insertion in the transgenic line of EPSPS6 with specific primer pair EPSPS6 F/P5. C: PCR confirmation of the T-DNA insertion in the transgenic line of EPS-7 with specific primer pair EPSPS7 F/P5. M: 2 kb DNA marker; P: pTGH-1 plasmid (positive control); N: J9707 (negative control); 1: T0-transgenic plant; 2-4: T1-transgenic plant; 5-7: T2-transgenic plant; 8-10: T3-transgenic plant.