新型抗广谱性除草剂草甘膦转基因油菜的创制及其鉴定

李杰华, 端群, 史明涛, 吴潞梅, 柳寒, 林拥军, 吴高兵, 范楚川, 周永明

Development and identification of transgenic rapeseed with a novel gene for glyphosate resistance

LI Jie-Hua, DUAN Qun, SHI Ming-Tao, WU Lu-Mei, LIU Han, LIN Yong-Jun, WU Gao-Bing, FAN Chu-Chuan, ZHOU Yong-Ming

图7 转I. variabilis EPSPS阳性株系的表达检测 A: RT-PCR检测目的基因在T0代株系叶片中的RNA表达水平。其中, M为3 kb DNA marker; 1~5依次为EPS-1、EPS-6、EPS-7、EPS-2和EPS-5等转化系; N: J9707 (阴性对照); P: pTGH-1质粒(阳性对照)。B: Western blot检测目的基因在T0代株系叶片中的蛋白表达水平。其中, N: J9707 (阴性对照); 1~6依次为EPS-1、EPS-6、EPS-36、EPS-7、EPS-2和EPS-5转化系。C: qRT-PCR检测目的基因在T3代株系的不同组织中相对表达量。油菜Actin2 (AF111812)为内参基因。

Fig. 7 Expression analysis of the transgenic lines of I. variabilis EPSPS A: the RNA expression levels of the target gene in the leaf of T0-positive transgenic lines detected by RT-PCR. M: 3 kb DNA marker; 1-5: the positive transgenic lines of EPS-1, EPS-6, EPS-7, EPS-2, and EPS-5, respectively; N: J9707 (negative control); P: pTGH-1 (positive control). B: the protein expression level of the target gene in the leaves of the T0-positive transgenic lines detected by Western blot. N: J9707 (negative control); 1-6: the positive transgenic lines of EPS-1, EPS-6, EPS-36, EPS-7, EPS-2, and EPS-5, respectively. C: the relative expression of the target gene in different tissues of the T3-positive transgenic lines detected by qRT-PCR. Actin2 (AF111812) in rape is an internal reference gene.