棉花GhMADS7基因正调控棉花花瓣发育

马欢欢, 方启迪, 丁元昊, 池华斌, 张献龙, 闵玲

GhMADS7 positively regulates petal development in cotton

MA Huan-Huan, FANG Qi-Di, DING Yuan-Hao, CHI Hua-Bin, ZHANG Xian-Long, MIN Ling

表1 本试验所用引物及用途

Table 1 Sequence and purpose of the primers used in this study

引物名称 Primer name 引物序列 Primer sequence (5°-3°) 用途 Purpose ubiquitin7-F GAAGGCATTCCACCTGACCAAC 实时荧光定量PCR ubiquitin7-R CTTGACCTTCTTCTTCTTGTGCTTG qRT-PCR GhAMDS7-S TCATAGTTCAATTCCTTGCCAGC 基因扩增 GhAMDS7-A CTTGAATAGATGGAGGTGGAAGAGAG Gene amplification OGhMADS7-F CCTTGCCAGCTTTGAACCAT 基因扩增 OGhMADS7-R ATGGAGGTGGAAGAGAGAAGCTAC Gene amplification qGhMADS7-F AATGATTACTCCAACCAAGACCAA 实时荧光定量PCR qGhMADS7-R TTGGTAGAACATTAGAGGCTGCTG qRT-PCR bpGhMADS7-F GGGGACAAGTTTGTACAAAAAAGCAGGCTGGCCTTGCCAGCTTTGAACCAT BP反应 bpGhMADS7-R GGGGACCACTTTGTACAAGAAAGCTGGGTCATGGAGGTGGAAGAGAGAAGCTAC BP reaction NPTII-F TTGTCACTGAAGCGGGAAGG 探针 NPTII-R CGATACCGTAAAGCACGAGGAA Probe