水稻单倍体诱导基因OsMATL突变体的创制与分析

文钦, 贾思思, 王加峰, 黄翠红, 王慧, 陈志强, 郭涛

Construction and identification of haploid induction gene OsMATL mutants in rice

WEN Qin, JIA Si-Si, WANG Jia-Feng, HUANG Cui-Hong, WANG Hui, CHEN Zhi-Qiang, GUO Tao

表1 本研究所用引物

Table 1 Primer sequences used in this study

引物名称 Primer name 引物序列Primer sequence (5'-3') OsMATL-P1-F GCATCCATGTGTTCCTGATCC OsMATL-P1-R GGTCCCATTAAGTTTGGCGG OsMATL-P2-F GGGAGAGTTGGGAACGTTGA OsMATL-P2-R TGTCGAGGTAGAAGCGGTTG Recom-F GTGCAGATGATCCGT GGCAACAAAGCACCAGTGGT Recom-R CTATTTCTAGCTCTAAAAC AAAAAAAAAAGCACCGACTCGGTG OsMATL-Cas9-1-F taGGTCTCCCTACCTCGACAAgttttagagctagaa OsMATL-Cas9-1-R cgGGTCTCAGTAGAAGCGGTTtgcaccagccgggaa OsMATL-Cas9-3-F taGGTCTCCAGGTCGAACTCGgttttagagctagaa OsMATL-Cas9-3-R cgGGTCTCAACCTCATCGACGtgcaccagccgggaa OsMATL-Cas9-4-F taGGTCTCCCAACGTCGAGACgttttagagctagaa OsMATL-Cas9-4-R cgGGTCTCAGTTGACCCTCGAtgcaccagccgggaa OsMATL-Cas9-P1-F taGGTCTCCTTGCTGGAAGGAgttttagagctagaa OsMATL-Cas9-P1-R cgGGTCTCAGCAAGGAAACTAtgcaccagccgggaa OsMATL-Cas9-P2-F taGGTCTCCTAATCCCATATTgttttagagctagaa OsMATL-Cas9-P2-R cgGGTCTCAATTAAACCCAGCtgcaccagccgggaa Cas9-J-F AAGCATTTCGTAGTGGGCCA Cas9-J-R TTTGGTGGTCGCCGTTAGGA OsMATL-1-F GCATCAGGGGCCTCATCC OsMATL-1-R GCCCAGCATCTTCCTGATCT OsMATL-3-F GCGTTGATTCCATCCGCATT OsMATL-3-R GCTCCTCCTTGTCCTTCACC OsMATL-4-F GCGCCCATCATCGACATCTT OsMATL-4-R TGCGTGTACAGTTACTAACGCT OsMATL-P-F GATCGATCGCCCATAAGCCA OsMATL-P-R TGACCAACTTGGCGTCATGA q-ACTIN-F GAATGCTAAGCCAAGAGGAG q-ACTIN-R AATCACAAGTGAGAACCACAG q-OsMATL-F CCGAGGTACAACGGCAAGTA q-OsMATL-R AGAAGATGGTTGGCTGGAGC