水稻单倍体诱导基因OsMATL突变体的创制与分析

文钦, 贾思思, 王加峰, 黄翠红, 王慧, 陈志强, 郭涛

Construction and identification of haploid induction gene OsMATL mutants in rice

WEN Qin, JIA Si-Si, WANG Jia-Feng, HUANG Cui-Hong, WANG Hui, CHEN Zhi-Qiang, GUO Tao

表2 T0代编码区纯合突变及启动子区大片段缺失突变类型

Table 2 Homozygous mutation in coding region and large fragment deletion mutation in promoter region types of T0 generation

植株编号 Number of plants 品种 Variety 序列 Sequence (5′-3′) 突变类型 Mutation type Exon 1 WT AACCGCTTCTACCTCGACAACGGCCCACTCATC 1-1-1 Nipponbare AACCGCTTCTACCTCGAACAACGGCCCACTCATC +1 bp 1-1-2 Nipponbare AACCGCTTCTACCTCGATCAACGGCCCACTCATC +1 bp 1-1-3 Nipponbare AACCGCTTCTACCTCGAATTCG --------------------------- ATC -8 bp and substitute 3 bp 1-1-4 Nipponbare AACCGCTT --------------------------------- AACGGCCCACTCATC -10 bp 1-2-1 Huahang 48 AACCGCTTCTACCTCGATCAACGGCCCACTCATC +1 bp Exon 3 WT CGTCGATGAGGTCGAACTCGCGGACCTTGCCGG 3-1-1 Nipponbare CGTCGATGAGGTCGAACTTCGCGGACCTTGCCGG +1 bp 3-1-2 Nipponbare CGTCGATGAGGTCGA-CTCGCGGACCTTGCCGG -1 bp 3-1-3 Nipponbare CGTCGATGAGGTCGAA-TCGCGGACCTTGCCGG -1 bp 3-1-4 Nipponbare CGTCGATGAGGTCGA ------ TCGCGGACCTTGCCGG -2 bp 3-1-5 Nipponbare CGTCGATGAGGTCG -------------------- CGGACCTTGCCGG -6 bp 3-1-6 Nipponbare CGTCGATGAGG ------------------------------ CGGACCTTGCCGG -9 bp 3-2-1 Huahang 48 CGTCGATGAGGTCGAAACTCGCGGACCTTGCCGG +1 bp 3-2-2 Huahang 48 CGTCGATGAGGTCGAA-TCGCGGACCTTGCCGG -1 bp 3-2-3 Huahang 48 CGTCGATGAGGTCGA ----- TCGCGGACCTTGCCGG -2 bp 3-2-4 Huahang 48 CGTCGATGAGGTC ------------ TCGCGGACCTTGCCGG -4 bp 3-2-5 Huahang 48 CGTCGATGAGGT --------------- TCGCGGACCTTGCCGG -5 bp Exon 4 WT TCGAGGGTCAACGTCGAGACCGGCAGGTACGTC 4-1-1 Nipponbare TCGAGGGTCAACGTCGAAGACCGGCAGGTACGTC +1 bp 4-1-2 Nipponbare TCGAGGGTCAACGTCGATGACCGGCAGGTACGTC +1 bp 4-1-3 Nipponbare TCGAGGGTCAACGTC-AGACCGGCAGGTACGTC -1 bp 4-1-4 Nipponbare TCGAGGGTCAACGTC ------- GACCGGCAGGTACGTC -2 bp 4-2-1 Huahang 48 TCGAGGGTCAACGTCGAAGACCGGCAGGTACGTC +1 bp 4-2-2 Huahang 48 TCGAGGGTCAACGTCGATGACCGGCAGGTACGTC +1 bp 4-2-3 Huahang 48 TCGAGGGTCAACGT ------- AGACCGGCAGGTACGTC -2 bp 4-2-4 Huahang 48 TCGAGGGTCAACGTC ------ GAACCGGCAGGTACGTC -2 bp 4-2-5 Huahang 48 TCGAGGGT --------------------------------------------------------------------- ACGTC -20 bp Promoter WT CCATCCTTCCAGCAAGGAAACTA(293 bp)CCGAATATGGGATTAAAC CCAGC P-1 Huahang 48 CCATCCT ------------------------------------- (-292 bp) -------------- ATGGGATTAAACCCAGC -292 bp P-2 Huahang 48 CCATCC ---------------------------------------- (-293 bp) ------------- ATGGGATTAAACCCAGC -293 bp P-3 Huahang 48 CCA -------------------------------------------------- (-304 bp) --------------------------------------- AAACCCAGC -304 bp P-4 Huahang 48 TTAGTTA -------------------------------------- (-306 bp) ------------------- GGGATTAAACCCAGC -306 bp P-5 Huahang 48 TTAGT --------------------------------------------- (-315 bp) ------------------------------------------ AACCCAGC -315 bp P-6 Huahang 48 TTAGT --------------------------------------------- (-363 bp) -------------------- AGGAGTATATAATTA -363 bp P-7 Huahang 48 CCAT ------------------------------------------------ (-722 bp) --------------------------------- TAGTTACGGAG -722 bp