甘蔗β-胡萝卜素异构酶基因家族的鉴定、定位和表达分析

黄宁, 惠乾龙, 方振名, 李姗姗, 凌辉, 阙友雄, 袁照年

Identification, localization and expression analysis of beta-carotene isomerase gene family in sugarcane

HUANG Ning, HUI Qian-Long, FANG Zhen-Ming, LI Shan-Shan, LING Hui, QUE You-Xiong, YUAN Zhao-Nian

表3 生物信息学分析结果

Table 3 Result of bioinformatics analysis

生物信息学分析 Bioinformatics analysis 结果Result 一级结构特性 Primary structure characteristics 氨基酸长度 Length of amino acids 266 等电点 Theoretical pI 8.91 分子量 Molecular weight (kD) 30.00 不稳定系数 Instability index (II) 56.40 二级结构类型 Secondary structure types α-螺旋 Alpha helix 30.08% 延伸链 Extended strand 12.41% 无规则卷曲 Random coil 57.52% 蛋白亚细胞定位 Protein subcellular location Plant-Ploc工具 Plant-Ploc tool 叶绿体Chloroplast ProtComp 9.0工具 ProtComp 9.0 tool 膜结合叶绿体Membrane bound Chloroplast WoLFPSORT工具 WoLFPSORT tool 叶绿体Chloroplast 功能位点 Function sites 靶向肽类型 Type of targeting peptide 叶绿体转运肽Chloroplast transit peptide 泛素化位点 Ubiquitination sites 4 磷酸化位点 Phosphorylation sites 18