甘蔗β-胡萝卜素异构酶基因家族的鉴定、定位和表达分析

黄宁, 惠乾龙, 方振名, 李姗姗, 凌辉, 阙友雄, 袁照年

Identification, localization and expression analysis of beta-carotene isomerase gene family in sugarcane

HUANG Ning, HUI Qian-Long, FANG Zhen-Ming, LI Shan-Shan, LING Hui, QUE You-Xiong, YUAN Zhao-Nian

图5 甘蔗栽培种ScD27.1基因的克隆与测序 A: ScD27.1基因的RT-PCR产物的琼脂糖电泳分析; M代表marker 100 bp-III DNA marker (Generay, 中国上海)。B: ScD27.1基因的开放读码框(*, 终止密码子)及其编码氨基酸序列, 粗体部分为特异性引物在基因序列中的位置。

Fig. 5 Cloning and sequencing of ScD27.1 gene in sugarcane cultivar A: agrose electrophoretic analysis of RT-PCR product of ScD27.1; M: 100 bp-III DNA marker (Generay, Shanghai, China). B: the open reading frame of ScD27.1 (*, stop codon) and its aa sequence; the sequence fragment complementary to primer is bolded.