小麦矮秆突变体的鉴定及其突变性状的关联分析

贺军与, 尹顺琼, 陈云琼, 熊静蕾, 王卫斌, 周鸿斌, 陈梅, 王梦玥, 陈升位

Identification of wheat dwarf mutants and analysis on association between the mutant traits of the dwarf plants

HE Jun-Yu, YIN Shun-Qiong, CHEN Yun-Qiong, XIONG Jing-Lei, WANG Wei-Bin, ZHOU Hong-Bin, CHEN Mei, WANG Meng-Yue, CHEN Sheng-Wei

表1 矮秆基因及其特异性标记的引物序列和片段长度

Table 1 Specific molecular markers of dwarf genes and the fragment length and primers sequence of the markers

基因 Gene 标记 Marker 正向引物 Forward sequence (5′-3′) 反向引物 Reverse sequence (5′-3′) 片段长度 Product size (bp) Rht-B1a BF-WR1 GGTAGGGAGGCGAGAGGCGAG CATCCCCATGGCCATCTCGAGCTG 237 Rht-B1b BF-MR1 GGTAGGGAGGCGAGAGGCGAG CATCCCCATGGCCATCTCGAGCTA 237 Rht-D1a BF2-WR2 GGCAAGCAAAAGCTTCGCG GGCCATCTCGAGCTGCAC 264 Rht-D1b BF-MR2 CGCGCAATTATTGGCCAGAGATAG CCCCATGGCCATCTCGAGCTGCTA 254 Rht4 WMC317 TGCTAGCAATGCTCCGGGTAAC TCACGAAACCTTTTCCTCCTCC 170 Rht5 BARC102 GGAGAGGACCTGCTAAAATCGAAGACA GCGTTTACGGATCAGTGTTGGAGA 200 Rht8 Xgwm261 CTCCCTGTACGCCTAAGGC CTCGCGCTACTAGCCATTG 192 Rht8 WMC503 GCAATAGTTCCCGCAAGAAAAG ATCAACTACCTCCAGATCCCGT 225 Rht9 BARC151 TGAGGAAAATGTCTCTATAGCATCC CGCATAAACACCTTCGCTCTTCCACTC 220 Rht12 WMC410 GGACTTGAAAGGAAGCTTGTGA CATGGATGGCATGCAGTGT 114 Rht13 WMS577 ATGGCATAATTTGGTGAAATTG TGTTTCAAGCCCAACTTCTATT 130 Rht14/16/18 XBARC3 TTCCCTGTGTCTTTCTAATTTTTTTT GCGAACTCCCGAACATTTTTAT 210