甘蓝型油菜温敏细胞核雄性不育系160S花药败育的形态学特征和细胞学研究

唐鑫, 李圆圆, 陆俊杏, 张涛

Morphological characteristics and cytological study of anther abortion of temperature-sensitive nuclear male sterile line 160S in Brassica napus

TANG Xin, LI Yuan-Yuan, LU Jun-Xing, ZHANG Tao

图5 160S-MF、160S-MS花药各时期细胞凋亡TUNEL检测 A: 160S-MF花粉母细胞时期; B: 160S-MS花粉母细胞时期; C: 160S-MF减数分裂时期; D和E: 160S-MS减数分裂时期; F: 160S-MF四分体时期; G: 160S-MF双核期; T: 绒毡层; PMC: 小孢子母细胞; Te: 四分体; M: 小孢子。碘化丙染色细胞核呈红色荧光, TUNEL阳性细胞核呈绿色荧光(箭头所指)。

Fig. 5 TUNEL detection of anther programmed cell dead at different developmental stages in 160S-MF and 160S-MS A: 160S-MF at pollen mother cell stage; B: 160S-MS at pollen mother cell stage; C: 160S-MF at meiotic stage; D and E:160S-MS at meiotic stage; F: 160S-MF at tetrad stage; G:160S-MF at binucleate stage; T: tapetum; PMC: pollen mother cell; Te: tetrad; M: microspore. The fluoresce of nuclei stained by propidium iodide is red, while green fluorescence is TUNEL-positive nuclei staining (arrow).