铅胁迫下红麻生理特性及DNA甲基化分析

李增强, 丁鑫超, 卢海, 胡亚丽, 岳娇, 黄震, 莫良玉, 陈立, 陈涛, 陈鹏

Physiological characteristics and DNA methylation analysis under lead stress in kenaf (Hibiscus cannabinus L.)

LI Zeng-Qiang, DING Xin-Chao, LU Hai, HU Ya-Li, YUE Jiao, HUANG Zhen, MO Liang-Yu, CHEN Li, CHEN Tao, CHEN Peng

表1 接头和引物序列

Table 1 Lists of adapter and primer sequences

引物类型 Primer type 引物名称及序列Primer name and sequence (5′-3′) EcoR I (E) Hpa II/Msp I (HM) 接头 Adapter EA1 CTCGTAGACTGCGTACC HMA1 GACGATGAGTCTAGAA EA2 AATTGGTACGCAGTC HMA2 CGTTCTAGACTCATC 预扩增 Pre-amplification E0 GACTGCGTACCAATTCA HM0 GATGAGTCTAGAACGGT E1 GACTGCGTACCAATTCAAC HM1 GATGAGTCTAGAACGGTAG E2 GACTGCGTACCAATTCAAG HM2 GATGAGTCTAGAACGGTAC E3 GACTGCGTACCAATTCACA HM3 GATGAGTCTAGAACGGTTG 选择性扩增 E4 GACTGCGTACCAATTCACT HM4 GATGAGTCTAGAACGGTTC Selective amplification E5 GACTGCGTACCAATTCACC HM5 GATGAGTCTAGAACGGTGT E6 GACTGCGTACCAATTCACG HM6 GATGAGTCTAGAACGGTGC E7 GACTGCGTACCAATTCAGC HM7 GATGAGTCTAGAACGGTCT E8 GACTGCGTACCAATTCAGG HM8 GATGAGTCTAGAACGGTCG