铅胁迫下红麻生理特性及DNA甲基化分析

李增强, 丁鑫超, 卢海, 胡亚丽, 岳娇, 黄震, 莫良玉, 陈立, 陈涛, 陈鹏

Physiological characteristics and DNA methylation analysis under lead stress in kenaf (Hibiscus cannabinus L.)

LI Zeng-Qiang, DING Xin-Chao, LU Hai, HU Ya-Li, YUE Jiao, HUANG Zhen, MO Liang-Yu, CHEN Li, CHEN Tao, CHEN Peng

表2 实时荧光定量PCR引物序列

Table 2 Primer sequences for qRT-PCR

引物名称 Primer name 正向引物 Forward sequence (5′-3′) 反向引物 Reverse sequence (5′-3′) AHL23 TCCTCCATCCGAACCAGTGTG CAAGGGAATCCAGAGAGACCATC CesA2 CCTAAAAATGCCGAACTCTACGC TAGAAAATCTGGACTCTCCTGGTGC ETO1 ATCTTTTCCGAGTTTGGTGTATCCT CGCTCCCTCCTTTCTTCGTC NPF5.4 TGAAGTTAGCAGCATCGGTTG TTCCAACACTTCCAAGAGGGT PLK TTGCGGCACTACGAGGTTGTT CCCCAATCATTCAATCTCCATACC PME7 CGCTGGACAAGAAAGATACCG GACACGCCGAGTGACATCATAGA RABA1f ACAAGGCTCACCATTTCCACAT GGGTCCCAGATACGAGGTTTTC SGT1 TCATTGCCAACACTAAAGCACACAT CCTAAGGCTACTCTGGAATCTGGGT VPS13F TTGAGCCATCGTGGTGAAGGG GCAGCAGGATTTGCGGTGTT WD40 ATGCCCTCCTTCAATGCGT CCCAATACCGATGAACAGCC β-man6 GATGATTGGTGGAGGAATGAGCA GTCGTCTGCTGCTTTAGAGGTCAC His3 (reference gene) GTGGAGTCAAGAAGCCTCACAG ATGGCTCTGGAAACGCAAA