铅胁迫下红麻生理特性及DNA甲基化分析
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李增强, 丁鑫超, 卢海, 胡亚丽, 岳娇, 黄震, 莫良玉, 陈立, 陈涛, 陈鹏
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Physiological characteristics and DNA methylation analysis under lead stress in kenaf (Hibiscus cannabinus L.)
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LI Zeng-Qiang, DING Xin-Chao, LU Hai, HU Ya-Li, YUE Jiao, HUANG Zhen, MO Liang-Yu, CHEN Li, CHEN Tao, CHEN Peng
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表2 实时荧光定量PCR引物序列
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Table 2 Primer sequences for qRT-PCR
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引物名称 Primer name | 正向引物 Forward sequence (5′-3′) | 反向引物 Reverse sequence (5′-3′) | AHL23 | TCCTCCATCCGAACCAGTGTG | CAAGGGAATCCAGAGAGACCATC | CesA2 | CCTAAAAATGCCGAACTCTACGC | TAGAAAATCTGGACTCTCCTGGTGC | ETO1 | ATCTTTTCCGAGTTTGGTGTATCCT | CGCTCCCTCCTTTCTTCGTC | NPF5.4 | TGAAGTTAGCAGCATCGGTTG | TTCCAACACTTCCAAGAGGGT | PLK | TTGCGGCACTACGAGGTTGTT | CCCCAATCATTCAATCTCCATACC | PME7 | CGCTGGACAAGAAAGATACCG | GACACGCCGAGTGACATCATAGA | RABA1f | ACAAGGCTCACCATTTCCACAT | GGGTCCCAGATACGAGGTTTTC | SGT1 | TCATTGCCAACACTAAAGCACACAT | CCTAAGGCTACTCTGGAATCTGGGT | VPS13F | TTGAGCCATCGTGGTGAAGGG | GCAGCAGGATTTGCGGTGTT | WD40 | ATGCCCTCCTTCAATGCGT | CCCAATACCGATGAACAGCC | β-man6 | GATGATTGGTGGAGGAATGAGCA | GTCGTCTGCTGCTTTAGAGGTCAC | His3 (reference gene) | GTGGAGTCAAGAAGCCTCACAG | ATGGCTCTGGAAACGCAAA |
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