剑麻苯丙氨酸裂解酶基因的鉴定及表达分析

黄兴, 习金根, 陈涛, 覃旭, 谭施北, 陈河龙, 易克贤

Identification and expression of PAL genes in sisal

HUANG Xing, XI Jin-Gen, CHEN Tao, QIN Xu, TAN Shi-Bei, CHEN He-Long, YI Ke-Xian

图1 拟南芥、水稻及龙舌兰属PAL基因遗传进化分析 At: 拟南芥; Ah: 剑麻; Am: 番麻; Ad: 沙漠龙舌兰; Aq: 太匮龙舌兰; Ao: 芦笋; Lus: 亚麻; Gorai: 棉花; Os: 水稻; GRMZM: 玉米; Bradi: 短柄草; Sobic: 高粱。利用ClustalX 2.0软件进行氨基酸序列的多重比对并构建Neighbor-Joining进化树(Bootstrap分析采用1000次重复)。

Fig. 1 Phylogenetic analysis of PALs in Arabidopsis, rice, and Agave species At: Arabidopsis thaliana; Ah: Agave H11648; Am: Agave americana; Ad: Agave deserti; Aq: Agave tequilana; Ao: Asparagus officinalis; Lus: Linum usitatissimum; Gorai: Gossypium raimondii; Os: Oryza sativa; GRMZM: Zea mays; Bradi: Brachypodium sylvaticum; Sobic: Sorghum bicolor. Multiple alignment of amino acid sequences was carried out and Neighbor-Joining evolutionary tree was constructed by ClustalX 2.0 software (Bootstrap values were tested for 1000 trails).