花生油脂合成相关基因的表达谱分析

许静, 潘丽娟, 李昊远, 王通, 陈娜, 陈明娜, 王冕, 禹山林, 侯艳华, 迟晓元

Expression pattern analysis of genes related to lipid synthesis in peanut

XU Jing, PAN Li-Juan, LI Hao-Yuan, WANG Tong, CHEN Na, CHEN Ming-Na, WANG Mian, YU Shan-Lin, HOU Yan-Hua, CHI Xiao-Yuan

表5 差异表达基因的RT-PCR验证结果

Table 5 RT-PCR validation results of the differentially expressed genes

基因编号 Gnen ID 基因注释 Gene annotation 发育时期 Development stage 调控a Regulation a 芯片数据(倍数) Microarray data (fold change) RT-PCR验证结果(倍数) RT-PCR result (fold change) AHTC1032413 黄酮醇合酶 DAP30 上调 6.05 13.64 Flavonol synthase flavanone 3-hydroxylase-like Up AHTC1030930 锌指蛋白 DAP30 下调 -3.78 -4.55 Dof zinc finger protein Down AHTC1005724 非特异性脂质转移酶 DAP40 上调 4.04 3.25 Non-specific lipid-transfer protein 3-like Up AHTC1030772 β-羟酰基-ACP脱水酶 DAP40 上调 2.86 2.11 Beta-hydroxyacyl-acp dehydrase 1 Up AHTC1020107 鞘脂Δ8 去饱和酶 DAP40 上调 2.04 3.48 Fatty acid sphingolipid desaturase Up AHTC1012845 WRI1 转录因子 DAP40 上调 2.15 2.31 Ethylene-responsive transcription factor wri1-like Up AHTC1030930 锌指蛋白 DAP40 下调 -5.30 -10.24 Dof zinc finger protein down AHTC1014100 肌醇半乳糖醇合酶 DAP40 下调 -3.09 -4.07 Galactinol synthase 8 Down AHTC1001812 β-酮酰基-ACP还原酶 DAP60 上调 7.89 5.09 Beta-ketoacyl-acp reductase 1-2 Up AHTC1001844 β-酮酰基-ACP合酶 DAP60 上调 4.49 4.83 Beta-ketoacyl-acp synthase i-2 Up AHTC1012845 WRI1 转录因子 DAP60 上调 5.83 21.21 Ethylene-responsive transcription factor wri1-like Up AHTC1005424 酰基载体蛋白硫酯酶 DAP60 上调 2.72 3.47 Oleoyl-acyl carrier protein thioesterase chloroplastic-like Up 基因编号 Gnen ID 基因注释 Gene annotation 发育时期 Development stage 调控a Regulationa 芯片数据(倍数) Microarray data (fold change) RT-PCR验证结果(倍数) RT-PCR result (fold change) AHTC1001749 硬脂酰-ACP去饱和酶 DAP60 上调 29.89 16.26 Stearoyl-acp desaturase Up AHTC1000715 ω6 脂肪酸去饱和酶 DAP60 上调 3.82 1.55 Omega 6 fatty acid desaturase Up AHTC1002482 天冬酰胺合成酶 DAP60 下调 -4.87 -6.12 Asparagine synthetase 2 Down AHTC1027178 Hsf 转录因子 DAP60 下调 -4.39 -9.85 Heat stress transcription factor a-2-like Down