玉米生长素响应因子家族基因的表达模式分析
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李文兰, 李文才, 孙琦, 于彦丽, 赵勐, 鲁守平, 李艳娇, 孟昭东
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A study of expression pattern of auxin response factor family genes in maize (Zea mays L.)
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LI Wen-Lan, LI Wen-Cai, SUN Qi, YU Yan-Li, ZHAO Meng, LU Shou-Ping, LI Yan-Jiao, MENG Zhao-Dong
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表1 实时定量PCR的引物序列
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Table 1 Primer sequences for real-time quantitative PCR
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基因名 Gene name | 基因号 Gene accession | 引物名称 Primer name | 引物序列 Primer sequence (5°-3°) | ARF1 | Zm00001 | arf1-s | CGTGTATATGTATCCTTCC | d030803 | arf1-a | ATTGTCTTCTGAGTACCA | ARF2 | Zm00001 | arf2-s | AAACGACTCTGGGTATGT | d031064 | arf2-a | CTGAAGGACTTGTGTCTG | ARF3 | Zm00001 | arf3-s | AACGGCATCTCTAACTAC | d031522 | arf3-a | ACGAATCTATGGAATTGAAC | ARF4 | Zm00001 | arf4-s | AGTTCCGATGGCAGTGTT | d032683 | arf4-a | CGAGGAACCGATGCAGAT | ARF5 | Zm00001 | arf5-s | CGCAACAACAACAGGCATG | d001879 | arf5-a | GGGCTAAAAAGGGACTGGTT | ARF6 | Zm00001 | arf6-s | AATCTCAGCAGCAGTTAA | d001945 | arf6-a | CTTGGGACTCTTGGTTTA | ARF7 | Zm00001 | arf7-s | CTAGTGACGCCCTGTACC | d003601 | arf7-a | AGTAATAGACACGCTCGC | ARF8 | Zm00001 | arf8-s | GTAGTTGAAGTGGATAATTGTT | d041056 | arf8-a | TCTGGAGAAGGCTGATTA | ARF9 | Zm00001 | arf9-s | TCTGAACCTCTGGTATCC | d041056 | arf9-a | AACAATTATCCACTTCAACTAC | ARF10 | Zm00001 | arf10-s | TGAACTCGAAATCAGCTGC | d042267 | arf10-a | AACTCCAACCTCCACTTGC | ARF11 | Zm00001d | arf11-s | ATGTTACAGGGAATGGGAATG | 043431 | arf11-a | CAACAGTTTGAGGAGCAGACG | ARF12 | Zm00001d | arf12-s | ATGGTAGACTTGATAGGA | 043922 | arf12-a | CATATTCACAGTTCCAGTA | ARF13 | Zm00001d | arf13-s | CAAGGCAATCACAATCTG | 049295 | arf13-a | CTGTCTGTTCATCCCAAA | ARF14 | Zm00001d | arf14-s | AATGACCGTTCTACTCCAATCA | 050781 | arf14-a | CTATCTCAATGCCAAACAATCT | ARF15 | Zm00001d | arf15-s | ATGAGGTCTTCGCCAGGAT | 051172 | arf15-a | GGACTGTGTCAGCGTCTTG | ARF16 | Zm00001d | arf16-s | AGAACATTGCTGATAGAT | 053819 | arf16-a | TTGTGTATGACCTTGAAT | ARF17 | Zm00001d | arf17-s | CCGTATATCCAAGGGTTTTG | 014013 | arf17-a | ATGTGGGGTCTCTTTATGTCA | ARF18 | Zm00001d | arf18-s | GCAGCAGATGGGGAAGCA | 014377 | arf18-a | AACTCGACCGAACCGACG | ARF19 | Zm00001d | arf19-s | AGAGGACGGCGGCAAGAT | 014507 | arf19-a | TGCTCGCCCTCGGGTAGT | ARF20 | Zm00001d | arf20-s | CCACCAATGAAGCAAGAA | 015243 | arf20-a | GATAGACAACATCTGACACAT | ARF21 | Zm00001d | arf21-s | AACAGAACAGCATTCAGT | 014690 | arf21-a | TGATTCAGTGGAAGAGATG | ARF22 | Zm00001d | arf22-s | GCTTTCCGCCAGCCTCA | 016838 | arf22-a | CCGGTGTCACCACCGATG | 基因名 Gene name | 基因号 Gene accession | 引物名称 Primer name | 引物序列 Primer sequence (5°-3°) | ARF23 | Zm00001d | arf23-s | CTGAGAGGACGGTGAGCAA | 000358 | arf23-a | CGCGACAGCCGAGAGGT | ARF24 | Zm00001d | arf24-s | GCCACTTTCAAGTCAGATT | 036593 | arf24-a | TTGGATTGTGCTCTCAGA | ARF25 | Zm00001d | arf25-s | CCCTCTTCTGTTCTTATGTTT | 038698 | arf25-a | TACTTCTTCACGGTTGGT | ARF26 | Zm00001d | arf26-s | GCATTCGCCCTCTTCTGTT | 038698 | arf26-a | AGGCTCGCTTCCATTTACA | ARF27 | Zm00001d | arf27-s | TTCCATCTCAATCCTCAT | 039006 | arf27-a | TCTATCCTCTTTCTTATTCAC | ARF28 | GRMZM2 | arf28-s | TTATGGTTCCAATACAAGAA | G075715 | arf28-a | CTCATTCCTATTCCTTAGC | ARF29 | Zm00001d | arf29-s | TTCAAGATCAGGGTTCAG | 011953 | arf29-a | GATTCAACCGTCAGAGAA | ARF30 | Zm00001d | arf30-s | CAAGTTCTTCAACATCAG | 045026 | arf30-a | ATCCTGTATTATGGTTCAA | ARF31 | Zm00001d | arf31-s | ACTCGCTGGGAAGAGGGCT | 023659 | arf31-a | CCTTTTGTCTGCTTCACCAC | ARF32 | Zm00001d | arf32-s | AGCTGGTGCGGGGCAAC | 025871 | arf32-a | CCTGCAAGGCCTCAATGAC | ARF33 | Zm00001d | arf33-s | AGTTGAATGCTCTTGGTA | 026540 | arf33-a | GTGAATCTGTGCTTCTTG | ARF34 | Zm00001d | arf34-s | ATGCTGGGTTGTTTGGTT | 026590 | arf34-a | GCGGCTAGAAAGTGGAAT | ARF35 | Zm00001d | arf35-s | ATGATATTGGAGCAGATG | 026687 | arf35-a | AAGAGCATTATGGTGTTC | 18S rRNA | | 18S-s | AAACGGCTACCACATCCAAG | | 18S-a | CCTCCAATGGATCCTCGTTA |
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