玉米光敏色素A1基因(ZmPHYA1)在棉花中的转化及分子鉴定

马燕斌, 王霞, 李换丽, 王平, 张建诚, 文晋, 王新胜, 宋梅芳, 吴霞, 杨建平

Transformation and molecular identification of maize phytochrome A1 gene (ZmPHYA1) in cotton

MA Yan-Bin, WANG Xia, LI Huan-Li, WANG Pin, ZHANG Jian-Cheng, WEN Jin, WANG Xin-Sheng, SONG Mei-Fang, WU Xia, YANG Jian-Ping

表1 转基因棉花植株中用于目标基因的检测引物

Table 1 PCR primers of objective genes in transgenic cotton

引物 Primers 序列 Primer sequences (5′-3′) 片段长度 Expected size (bp) Basta-F ACCATCGTCAACCACTACATC 256 Basta-R GCTGCCAGAAA CCCACGTCAT ZmPHYA1-1641F CAGCAGAAGGATGCACCCTAGGCTG 217 ZmPHYA1-1857R CGCTTGCAGTTCGGCGAGCCCATCA