玉米光敏色素A1基因(ZmPHYA1)在棉花中的转化及分子鉴定

马燕斌, 王霞, 李换丽, 王平, 张建诚, 文晋, 王新胜, 宋梅芳, 吴霞, 杨建平

Transformation and molecular identification of maize phytochrome A1 gene (ZmPHYA1) in cotton

MA Yan-Bin, WANG Xia, LI Huan-Li, WANG Pin, ZHANG Jian-Cheng, WEN Jin, WANG Xin-Sheng, SONG Mei-Fang, WU Xia, YANG Jian-Ping

图1 外源ZmPHYA1 基因的棉花转化与再生植株的草铵膦抗性基因检测 A: 农杆菌侵染后的愈伤筛选及诱导; B: 愈伤诱导增殖; C: 胚型愈伤分化形成的体细胞胚胎; D: 再生的幼苗; E: 再生的嫁接植株; F: 部分再生株草铵膦抗性基因的检测, 其中1、2、4分别为株系Line 9、Line 14和Line 41, 3为非转基因的再生株, “-”为受体对照, “+”为质粒阳性扩增, M: DNA marker。

Fig. 1 Transformation of exogenous ZmPHYA1 gene and the PCR detection of glufosinate-resistance genes in cotton plants A: callus screening and induction of cotton hypocotyl after Agrobacterium infection; B: callus induction and proliferation; C: somatic embyos formed from embryonic callus; D: regeneration plants; E: survived regenerated graft plants; F: PCR detection of glufosinate-resistance gene in some regenerated plants. Among them, Lane 1, 2 and 4 denote Line 9, Line 14, and Line 41, respectively, 3 denotes the non-transgenic regenerated plant, “-” denotes WT control, and “+” denotes plasmid positive amplification. M: DNA marker.