玉米光敏色素A1基因(ZmPHYA1)在棉花中的转化及分子鉴定

马燕斌, 王霞, 李换丽, 王平, 张建诚, 文晋, 王新胜, 宋梅芳, 吴霞, 杨建平

Transformation and molecular identification of maize phytochrome A1 gene (ZmPHYA1) in cotton

MA Yan-Bin, WANG Xia, LI Huan-Li, WANG Pin, ZHANG Jian-Cheng, WEN Jin, WANG Xin-Sheng, SONG Mei-Fang, WU Xia, YANG Jian-Ping

图2 转外源基因ZmPHYA1棉花草铵膦抗性株系的鉴定与PCR分子检测 A, B: 抗除草剂检测的不同阳性植株后代; C: 分离的非抗性植株后代; D: 部分抗性植株的ZmPHYA1 基因的PCR检测, 其中“+”为质粒阳性扩增, 1和2为Line 9株系的不同后代单株, 3和4为Line 14株系的不同后代单株, 5和6为Line 41株系的不同后代单株。“-”为受体对照; M: DNA marker。

Fig. 2 Glufosinate resistant identification and PCR detection of exogenous ZmPHYA1 gene in cotton A, B: different positive plant offspring detected for herbicide resistance; C: isolated offspring of non-resistant plants; D: PCR detection of ZmPHYA1 in some resistant plants. Among them, “+” denotes plasmid positive amplification, 1 and 2 denote single progeny plant of Line 9, 3 and 4 denote single progeny plant of Line 14, 5 and 6 denote single progeny plant of Line 41. “-” denotes WT; M: DNA marker.