玉米光敏色素<i>A1</i>基因(<i>ZmPHYA1</i>)在棉花中的转化及分子鉴定

玉米光敏色素A1基因(ZmPHYA1)在棉花中的转化及分子鉴定

马燕斌, 王霞, 李换丽, 王平, 张建诚, 文晋, 王新胜, 宋梅芳, 吴霞, 杨建平

Transformation and molecular identification of maize phytochrome A1 gene (ZmPHYA1) in cotton

MA Yan-Bin, WANG Xia, LI Huan-Li, WANG Pin, ZHANG Jian-Cheng, WEN Jin, WANG Xin-Sheng, SONG Mei-Fang, WU Xia, YANG Jian-Ping

图3 不同棉花转基因株系中外源ZmPHYA1蛋白表达的免疫印迹检测
M: 蛋白marker; WT: 转基因棉花受体R15; Isolated non-transgenic line: 转基因株系分离的非抗性株; #9、#14、#41分别为转外源ZmphyA1基因的不同株系Line 9、Line 14和Line 41。“+”为检测中利用转玉米中同源基因ZmPHYA2 (3393 bp)的阳性棉花植株。

Fig. 3 Immuno-blot detection of exogenous ZmPHYA1 protein expression in transgenic cotton lines
M: protein markers; WT: transgenic cotton receptor R15; non-transgenic line: non-resistant plants isolated from transgenic lines. Lane #9, #14, #41 represent Line 9, Line 14 and Line 41 of transformed exogenous ZmPHYA1 gene plants. “+” is positive cotton plant using the homologous gene ZmPHYA2 (3393 bp) in the transformed maize.