基于SSR标记的雪茄烟种质资源指纹图谱库的构建及遗传多样性分析

王琰琰, 王俊, 刘国祥, 钟秋, 张华述, 骆铮珍, 陈志华, 戴培刚, 佟英, 李媛, 蒋勋, 张兴伟, 杨爱国

Construction of SSR fingerprint database and genetic diversity analysis of cigar germplasm resources

WANG Yan-Yan, WANG Jun, LIU Guo-Xiang, ZHONG Qiu, ZHANG Hua-Shu, LUO Zheng-Zhen, CHEN Zhi-Hua, DAI Pei-Gang, TONG Ying, LI Yuan, JIANG Xun, ZHANG Xing-Wei, YANG Ai-Guo

表1 SSR 引物信息

Table 1 Information of SSR markers

引物 Primer 上游引物 Forward primer (5'-3') 下游引物 Reverse primer (5'-3') 基因组 Group 位置 Position PT52940 TCTTAGCCGTCCATCTCTGC CACAGGACAATTTGTTGCAGTT 7 134.635 PT52118 ATCCCAAGCAAGTTGGTGTC AACGAGCATTATGGGATTGC 18 70.312 PT51894 AACAACAGCGGATCACATTG TGTATGCAACCCACAGAACAA 8 78.215 PT30163 CAACACACACGCTCATCAGA TCCTCATCCATCGGTAAAGG 5 12.216 PT50433 TTTGTAAATTGTGTGTATAGAGGAGAG GCACTGGATCTCCTGCATTT 16 160.451 PT51005 CCCTTCGTTCTACGAACCCT GGGTGCCACCTCACTTTCT 10 55.231 PT51145 ATGCAAATGCAAGATGGTCA CCGTTCAACTTGGCTTTACC 3 103.357 PT51234 AATTGGACCCAACCACTCTG TAGCAGGGAAGCCGAAAGTA 19 66.109 PT51289 GAGTTGTGGCCAAGTAGCCT GGCTTTAGCCAAACGCTCTA 21 49.700 PT51420 CGCCATTACGAACACTAGCA AAGTTTAGTCCGGTCCGGTT 16 73.595 PT52821 GGGCAATGGAGCAATAGAAA TGCTTAAAGATTTCCACTCGTT 20 95.174 PT53857 TTCATAAATCAACCTTAAGTGGAGC TTTGGAGCTTGAAAGCAAAGA 22 67.708 PT54027 GGATCTGCTCATTTCCTATGG CAAGGCAGCTAAGCCTAACAA 11 50.950 PT54444 GTGCCCATACCAGATTGTGA ATTCTTCCAACATTCGCACC 6 54.463 PT55056 GCTCTTTAGTCTTTAGGGAGTTGG TGAATTGAGCTCCTTCCAGG 14 0.276 PT60037 TGGTCTTTGTTCACCACCAA GCTGGTTCCATCAATTCCAT 4 88.053 PT60146 AAACCTGGAAAGCAACATCA GACCACGTTGTGTAGTTGTATTCTT 15 45.400 PT60379 AATTTGCACTCTGCGGACTC CCCTGGGAGTTTCCATTTCT 14 28.140 PT60726 GCAGAGGCAACAATGCATAC AGCTCGTTGCTGTTTCATCA 20 2.521 PT60990 TCAACCCATAAAGCTGCTCC AAAGAGACAAAGCAGGCACAA 23 4.978 PT61187 GCTCAGTCGTGAAGAAACAGAA AGGGAATCCTTGGTTGGTTT 5 130.242 PT61220 TTGGCATGGTTGTGTAAGGA GCCACAAGTTATAATTTAAAGCTACCA 6 102.936 PT61226 CCTTGGACTCACAATACCATCA CCTGTTAGCCAAAGGGTAATTC 13 46.719 PT30272 GAACCTAACCTCGCTCCACA AAATGGTAGCTGCGAGGAGA 15 106.325 PT60427 TGATTCCCTAACCTGCGAAC CCATTCTTGAGCTTCGTTCTTT 21 5.867 PT51378 ATGTCGGGAAGATTCCACAG ACAACCAGCTAAATGTGCCC 17 111.136 PT1245 AGTTTAATGTGGGAGCGA TCGGTCGGTATTAGTGGT 11 39.717 PT50736 CCAAGTAGCGACGAGGAGAA TTATCCACGCGTCACCATTA 24 53.932 PT51059 CAAACCAGCGCATCTGTCTA CACCAGAACCCGCTAGTCTC 18 15.309 PT51144 ACCGACAACACACGATTGTA GCTTGTCGCTTAGCTTAACCAT 10 24.533 PT51878 TGGGAAGAGTGCTACCTTAGTACA GCGATGTACTCCTAAAGATGTCAA 24 60.520 PT51976 CAGAGGGTAGTGAAGTATTCAATTT GTTATCAATGCCCTGCCCT 22 94.306 PT52088 TGTGATTCGAACTTGTGATCCT CATCAGTGGAAAGCGACTCA 7 87.079 PT52156 TGAGAATTCATATAACCGACCCA TGCCTTCTTTCATACTGCCA 23 60.339 PT52176 TTGCATAAGAGTACCCATCTCG TGTGTGTGTGAAGCTTGAAGAA 9 120.275 PT52689 GCACCAGGTGAAGTAAAGACAA TGAAATGCATGTAAAGGAAAGG 13 66.991 PT53216 TCCAAACTCTTATCCCTGATCC CACCTACCCTGTCGCTGTTT 2 0 PT54394 CATCTCAACTCGCCTGACAA GCGGCAGGGTTCTATGATTA 8 96.735 PT54889 GGGTCTAAATAATCGCAATTGAA CGCAGGTGGACTTAAAGAATC 19 106.147 PT60080 CTACGCAGAATCCAATTCCA CATTAGGCCATAGCCATCCA 3 179.150 PT60917 CAGGAGACTCCATAAATTCACTCTT AGCATGGTTGCTATTGGCTG 9 38.417 PT61025 GAACCAATCCAAGAGCCAGA TCAGCCAAATATGTAGCAGGTG 1 87.176 PT61343 ACCCATTTAATTGCCACGTC TATTCCGGCAGGAGTTTGAC 1 22.254