基于SSR标记的雪茄烟种质资源指纹图谱库的构建及遗传多样性分析

王琰琰, 王俊, 刘国祥, 钟秋, 张华述, 骆铮珍, 陈志华, 戴培刚, 佟英, 李媛, 蒋勋, 张兴伟, 杨爱国

Construction of SSR fingerprint database and genetic diversity analysis of cigar germplasm resources

WANG Yan-Yan, WANG Jun, LIU Guo-Xiang, ZHONG Qiu, ZHANG Hua-Shu, LUO Zheng-Zhen, CHEN Zhi-Hua, DAI Pei-Gang, TONG Ying, LI Yuan, JIANG Xun, ZHANG Xing-Wei, YANG Ai-Guo

表2 43对SSR在220份材料中的遗传多样性参数

Table 2 Genetic diversity of 43 SSR makers in 220 cigar germplasm resources

引物 Primer 等位基 因数 Number of alleles (Na) 有效等位 基因数 Number of effective alleles (Ne) Shannon’s 多样性指数 Shannon’s diversity index (I) 观测杂合度Observed heterozygosity (Ho) 期望杂合度Expected heterozygosity (He) Nei’s 多样性指数 Nei’s diversity index (H) 多态性信息含量 Polymorphism information content (PIC) PT52940 6 3.6152 1.3638 0.2750 0.7250 0.7234 0.6729 PT52118 4 2.9357 1.2000 0.3391 0.6609 0.6594 0.6019 PT51894 4 3.7568 1.3501 0.2645 0.7355 0.7338 0.6838 PT30163 4 2.2131 1.0189 0.4506 0.5494 0.5481 0.5017 PT50433 5 2.6611 1.0961 0.3743 0.6257 0.6242 0.5495 PT51005 7 5.0382 1.7676 0.1966 0.8034 0.8015 0.7760 PT51145 4 3.4657 1.3172 0.2869 0.7131 0.7115 0.6634 PT51234 4 1.5812 0.7298 0.6316 0.3684 0.3676 0.3415 PT51289 4 3.7381 1.3521 0.2658 0.7342 0.7325 0.6838 PT51420 4 2.9343 1.1847 0.3393 0.6607 0.6592 0.5943 PT52821 6 3.4023 1.3790 0.2923 0.7077 0.7061 0.6543 PT53857 6 1.8837 0.8822 0.5298 0.4702 0.4691 0.4117 PT54027 8 4.2139 1.6993 0.2355 0.7645 0.7627 0.7364 PT54444 3 2.4175 0.9805 0.4123 0.5877 0.5864 0.5170 PT55056 4 2.5613 1.1245 0.3890 0.6110 0.6096 0.5573 PT60037 5 2.4156 1.0418 0.4126 0.5874 0.5860 0.5153 PT60146 6 3.7451 1.4714 0.2653 0.7347 0.7330 0.6907 PT60379 6 4.4270 1.5781 0.2240 0.7760 0.7741 0.7375 PT60726 4 2.7073 1.1729 0.3679 0.6321 0.6306 0.5829 PT60990 5 4.3975 1.5329 0.2255 0.7745 0.7726 0.7352 PT61187 7 6.0233 1.8663 0.1641 0.8359 0.8340 0.8126 PT61220 6 4.8084 1.6639 0.2061 0.7939 0.7920 0.7621 PT61226 9 5.9444 1.9354 0.1663 0.8337 0.8318 0.8108 PT30272 7 5.6551 1.8289 0.1749 0.8251 0.8232 0.7999 PT60427 6 3.7898 1.5358 0.2622 0.7378 0.7361 0.7020 PT51378 7 5.5863 1.8098 0.1771 0.8229 0.8210 0.7969 PT1245 4 2.6871 1.0545 0.3707 0.6293 0.6279 0.5518 PT50736 3 2.1267 0.8116 0.4690 0.5310 0.5298 0.4192 PT51059 6 1.4182 0.6834 0.7045 0.2955 0.2949 0.2854 PT51144 8 5.2021 1.7598 0.1904 0.8096 0.8078 0.7804 PT51878 4 3.8172 1.3605 0.2603 0.7397 0.7380 0.6891 PT51976 9 6.7316 1.9878 0.1466 0.8534 0.8514 0.8335 PT52088 3 1.7058 0.7370 0.5853 0.4147 0.4138 0.3724 PT52156 4 2.0324 0.7439 0.4909 0.5091 0.5080 0.3878 PT52176 2 1.3081 0.3983 0.7639 0.2361 0.2355 0.2078 PT52689 13 11.7876 2.5112 0.0828 0.9172 0.9152 0.9087 PT53216 5 4.7386 1.5826 0.2092 0.7908 0.7890 0.7553 PT54394 4 2.1517 0.9554 0.4635 0.5365 0.5352 0.4776 PT54889 7 5.8341 1.8356 0.1695 0.8305 0.8286 0.8054 PT60080 10 7.0791 2.0931 0.1393 0.8607 0.8587 0.8433 PT60917 9 6.4282 1.9900 0.1536 0.8464 0.8444 0.8264 PT61025 5 2.7206 1.1786 0.3661 0.6339 0.6324 0.5793 PT61343 6 4.3419 1.5823 0.2285 0.7715 0.7697 0.7340 平均值Mean 5.6512 3.9077 1.3756 0.3191 0.6809 0.6793 0.6360